Real-time fluorescence assays of alkaline phosphatase and ATP sulfurylase activities based on a novel PPi fluorescent probe

被引：27

作者：

Wang, Xiaobo ^{[1
]}

Zhang, Zhiyang ^{[1
]}

Ma, Xiaoyan ^{[1
]}

Wen, Jinghan ^{[1
]}

Geng, Zhirong ^{[1
]}

Wang, Zhilin ^{[1
]}

机构：

[1] Nanjing Univ, Sch Chem & Chem Engn, Collaborat Innovat Ctr Adv Microstruct, State Key Lab Coordinat Chem, Nanjing 210093, Jiangsu, Peoples R China

来源：

TALANTA | 2015年 / 137卷

基金：

中国国家自然科学基金;

关键词：

PPi; Fluorescent probe; Alkaline phosphatase; ATP sulfurylase; Real-time fluorescence assay; ADENOSINE 5'-PHOSPHOSULFATE KINASE; RHEUMATOID-ARTHRITIS; INORGANIC PYROPHOSPHATE; MECHANISM; ARABIDOPSIS; HYDROLYSIS; METABOLISM; BIOSENSOR; DISEASE; STEP;

D O I：

10.1016/j.talanta.2015.01.028

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An anthracene-armed tetraaza macrocyclic fluorescent probe 3-(9-anthrylmethyl)-3,6,9,15-tetraazabicyclo[9.3.1]pentadeca-1(15),11,13-triene(L) for detecting Zn2+ in aqueous medium was synthesized. L-Zn2+ complex, showed selectivity toward pyrophosphate ion (PPi) by quenching the fluorescence in aqueous HEPES buffer (pH 7.4). Furthermore, L-Zn2+ was also used to set up a real-time fluorescence assay for monitoring enzyme activities of alkaline phosphatase (ALP) and adenosine triphosphate sulfurylase (ATPS). In the presence of ALP inhibitor Na3VO4 and ATPS inhibitor chlorate, two enzymes activities decreased obviously, respectively. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：156 / 160

页数：5