Turn On of a Ruthenium(II) Photocatalyst by DNA-Templated Ligation

被引：22

作者：

Anzola, Marcello ^{[1
]}

Winssinger, Nicolas ^{[1
]}

机构：

[1] Univ Geneva, Fac Sci, Dept Organ Chem, NCCR Chem Biol, 30 Quai Ernest Ansermet, CH-1205 Geneva, Switzerland

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2019年 / 25卷 / 01期

基金：

瑞士国家科学基金会;

关键词：

DNA; photocatalysis; ruthenium; SIGNAL AMPLIFICATION; NUCLEIC-ACIDS; LIVE CELLS; COMPLEXES; FREQUENCY; SUBSTRATE; CHEMISTRY; QUENCHER; PROBES; MIRNA;

D O I：

10.1002/chem.201804283

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Here, the synthesis of a Ru-II photocatalyst by light-directed oligonucleotide-templated ligation reaction is described. The photocatalyst was found to have tremendous potential for signal amplification with >15000 turnovers measured in 9 hours. A templated reaction was used to turn on the activity of this ruthenium(II) photocatalyst in response to a specific DNA sequence. The photocatalysis of the ruthenium(II) complex was harnessed to uncage a new precipitating dye that is highly fluorescent and photostable in the solid state. This reaction was used to discriminate between different DNA analytes based on localization of the precipitate as well as for in cellulo miRNA detection. Finally, a bipyridine ligand functionalized with two different peptide nucleic acid (PNA) sequences was shown to enable template-mediated ligation (turn on of the ruthenium(II) photocatalysis) and recruitment of substrate for templated photocatalysis.

引用

页码：334 / 342

页数：9

共 52 条

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