Review of applications of CRISPR-Cas9 gene-editing technology in cancer research

被引：21

作者：

Zhao, Ziyi ^{[1
]}

Li, Chenxi ^{[1
]}

Tong, Fei ^{[2
]}

Deng, Jingkuang ^{[1
]}

Huang, Guofu ^{[1
]}

Sang, Yi ^{[1
]}

机构：

[1] Nanchang Univ, Affiliated Hosp 3, Nanchang 330008, Jiangxi, Peoples R China

[2] Nanchang Univ, Affiliated Stomatol Hosp, Orthodont Dept, Nanchang 330008, Jiangxi, Peoples R China

来源：

BIOLOGICAL PROCEDURES ONLINE | 2021年 / 23卷 / 01期

关键词：

CRISPR; Cas9; Cancer; High throughput screening; Cancer treatment; GENOMIC DNA; MOUSE MODEL; CRISPR/CAS9; RNA; TARGET; CAS; DISCOVERY; SCREENS; CELLS; REARRANGEMENTS;

D O I：

10.1186/s12575-021-00151-x

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Characterized by multiple complex mutations, including activation by oncogenes and inhibition by tumor suppressors, cancer is one of the leading causes of death. Application of CRISPR-Cas9 gene-editing technology in cancer research has aroused great interest, promoting the exploration of the molecular mechanism of cancer progression and development of precise therapy. CRISPR-Cas9 gene-editing technology provides a solid basis for identifying driver and passenger mutations in cancer genomes, which is of great value in genetic screening and for developing cancer models and treatments. This article reviews the current applications of CRISPR-Cas9 gene-editing technology in various cancer studies, the challenges faced, and the existing solutions, highlighting the potential of this technology for cancer treatment.

引用

页数：13

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