A Structural Study of Escherichia coli Cells Using an In Situ Liquid Chamber TEM Technology

被引：12

作者：

Wang, Yibing ^{[1
]}

Chen, Xin ^{[2
,3
,4
]}

Cao, Hongliang ^{[2
,3
]}

Deng, Chao ^{[2
,3
]}

Cao, Xiaodan ^{[1
]}

Wang, Ping ^{[1
]}

机构：

[1] E China Univ Sci & Technol, Biomed Nanotechnol Ctr, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

[2] E China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China

[3] E China Univ Sci & Technol, Sch Mat Sci & Engn, Shanghai Key Lab Adv Polymer Mat, Shanghai 200237, Peoples R China

[4] Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, State Key Lab Funct Mat Informat, Shanghai 200050, Peoples R China

来源：

JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY | 2015年 / 2015卷

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

WET-CELL; ELECTRON-MICROSCOPY; TRANSMISSION; NANOPARTICLES; ARCHITECTURE;

D O I：

10.1155/2015/829302

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Studying cell microstructures and their behaviors under living conditions has been a challenging subject in microbiology. In this work, in situ liquid chamber TEM was used to study structures of Escherichia coli cells in aqueous solutions at a nanometer-scale resolution. Most of the cells remained intact under electron beam irradiation, and nanoscale structures were observed during the TEM imaging. The analysis revealed structures of pili surrounding the E. coli cells; themovements of the pili in the liquid were also observed during the in situ tests. This technology also allowed the observation of features of the nucleoid in the E. coli cells. Overall, in situ TEM can be applied as a valuable tool to study real-time microscopic structures and processes in microbial cells residing in native aqueous solutions.

引用

页数：7

共 27 条

[1] In situ electrochemical wet cell transmission electron microscopy characterization of solid-liquid interactions between Ni and aqueous NiCl2 [J].

Chen, X. ;

Noh, K. W. ;

Wen, J. G. ;

Dillon, S. J. .

ACTA MATERIALIA, 2012, 60 (01) :192-198

[2] A Study of Nano Materials and Their Reactions in Liquid Using in situ Wet Cell TEM Technology [J].

Chen Xin ;

Zhou Lihui ;

Wang Ping ;

Zhao Chongjun ;

Miao Xiaoli .

CHINESE JOURNAL OF CHEMISTRY, 2012, 30 (12) :2839-2843

[3] In situ wet-cell TEM observation of gold nanoparticle motion in an aqueous solution [J].

Chen, Xin ;

Wen, Jianguo .

NANOSCALE RESEARCH LETTERS, 2012, 7 :1-6

[4] Electron microscopy of whole cells in liquid with nanometer resolution [J].

de Jonge, N. ;

Peckys, D. B. ;

Kremers, G. J. ;

Piston, D. W. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (07) :2159-2164

[5] Electron energy-loss spectroscopy in the TEM [J].

Egerton, R. F. .

REPORTS ON PROGRESS IN PHYSICS, 2009, 72 (01)

[6] Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy [J].

Evans, James E. ;

Jungjohann, Katherine L. ;

Wong, Peony C. K. ;

Chiu, Po-Lin ;

Dutrow, Gavin H. ;

Arslan, Ilke ;

Browning, Nigel D. .

MICRON, 2012, 43 (11) :1085-1090

[7] In situ imaging of orthoclase-aqueous solution interfaces with x-ray reflection interface microscopy [J].

Fenter, P. ;

Lee, S. S. ;

Zhang, Z. ;

Sturchio, N. C. .

JOURNAL OF APPLIED PHYSICS, 2011, 110 (10)

[8] Role of water in electron-initiated processes and radical chemistry: Issues and scientific advances [J].

Garrett, BC ;

Dixon, DA ;

Camaioni, DM ;

Chipman, DM ;

Johnson, MA ;

Jonah, CD ;

Kimmel, GA ;

Miller, JH ;

Rescigno, TN ;

Rossky, PJ ;

Xantheas, SS ;

Colson, SD ;

Laufer, AH ;

Ray, D ;

Barbara, PF ;

Bartels, DM ;

Becker, KH ;

Bowen, H ;

Bradforth, SE ;

Carmichael, I ;

Coe, JV ;

Corrales, LR ;

Cowin, JP ;

Dupuis, M ;

Eisenthal, KB ;

Franz, JA ;

Gutowski, MS ;

Jordan, KD ;

Kay, BD ;

LaVerne, JA ;

Lymar, SV ;

Madey, TE ;

McCurdy, CW ;

Meisel, D ;

Mukamel, S ;

Nilsson, AR ;

Orlando, TM ;

Petrik, NG ;

Pimblott, SM ;

Rustad, JR ;

Schenter, GK ;

Singer, SJ ;

Tokmakoff, A ;

Wang, LS ;

Wittig, C ;

Zwier, TS .

CHEMICAL REVIEWS, 2005, 105 (01) :355-389

[9] Exploring the 3D molecular architecture of Escherichia coli type 1 pili [J].

Hahn, E ;

Wild, P ;

Hermanns, U ;

Sebbel, P ;

Glockshuber, R ;

Häner, M ;

Taschner, N ;

Burkhard, P ;

Aebi, U ;

Müller, SA .

JOURNAL OF MOLECULAR BIOLOGY, 2002, 323 (05) :845-857

[10] Damage of the Bacterial Cell Envelope by Antimicrobial Peptides Gramicidin S and PGLa as Revealed by Transmission and Scanning Electron Microscopy [J].

Hartmann, Mareike ;

Berditsch, Marina ;

Hawecker, Jacques ;

Ardakani, Mohammad Fotouhi ;

Gerthsen, Dagmar ;

Ulrich, Anne S. .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2010, 54 (08) :3132-3142

← 1 2 3 →