Effective Targeting of DC-SIGN by α-Fucosylamide Functionalized Gold Nanoparticles

被引：51

作者：

Arosio, Daniela ^{[1
]}

Chiodo, Fabrizio ^{[2
,3
]}

Reina, Jose J. ^{[4
]}

Marelli, Marcello ^{[1
]}

Penades, Soledad ^{[2
,5
]}

van Kooyk, Yvette ^{[3
]}

Garcia-Vallejo, Juan J. ^{[3
]}

Bernardi, Anna ^{[1
,4
]}

机构：

[1] CNR Inst Mol Sci & Technol ISTM, I-20133 Milan, Italy

[2] CIC biomaGUNE, Biofunct Nanomat Unit, Lab GlycoNanotechnol, San Sebastian 20009, Spain

[3] Vrije Univ Amsterdam Med Ctr, Dept Mol Cell Biol & Immunol, NL-1081 BT Amsterdam, Netherlands

[4] Univ Milan, Dipartimento Chim, I-20133 Milan, Italy

[5] CIBER BBN, San Sebastian 20009, Spain

来源：

BIOCONJUGATE CHEMISTRY | 2014年 / 25卷 / 12期

关键词：

HUMAN DENDRITIC CELLS; LECTIN RECEPTORS; IMMUNE-RESPONSES; T-CELLS; LIGANDS; ICAM-3; GLYCONANOPARTICLES; SPECIFICITY; NONINTEGRIN; SYSTEMS;

D O I：

10.1021/bc500467u

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Dendritic Cells (DCs), the most potent antigen-presenting cells, play a critical role in the detection of invading pathogens, which are recognized also by multiple carbohydrate-specific receptors. Among them, DC-SIGN is one of the best characterized, with high-mannose and Lewis-type glycan specificity. In this study, we present a potent DC-SIGN targeting device developed using gold nanoparticles functionalized with a-fucosyl-beta-alanyl amide. The nanoparticles bound to cellular DC-SIGN and induced internalization as effectively as similar particles coated with comparable amounts of Lewis(X) oligosaccharide. They were found to be neutral toward dendritic cell maturation and IL-10 production, thus envisaging a possible use as targeted imaging tools and antigen delivery devices.

引用

页码：2244 / 2251

页数：8

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