MiR-17-5p and MKL-1 modulate stem cell characteristics of gastric cancer cells

被引：24

作者：

Dai, Zhou-Tong ^{[1
]}

Xiang, Yuan ^{[2
]}

Duan, Yuan-yuan ^{[1
]}

Wang, Jun ^{[1
]}

Li, Jia Peng ^{[1
]}

Zhang, Hui-Min ^{[1
]}

Cheng, Chao ^{[3
]}

Wang, Qiong ^{[1
]}

Zhang, Tong-Cun ^{[1
,4
]}

Liao, Xing-Hua ^{[1
]}

机构：

[1] Wuhan Univ Sci & Technol, Coll Life & Hlth Sci, Inst Biol & Med, Wuhan 430081, Hubei, Peoples R China

[2] Huazhong Univ Sci & Technol, Cent Hosp Wuhan, Tongji Med Coll, Dept Med Lab, Wuhan 430014, Hubei, Peoples R China

[3] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Gastrointestinal Surg, Wuhan, Hubei, Peoples R China

[4] Tianjin Univ Sci & Technol, Coll Biotechnol, Key Lab Ind Fermentat Microbiol, Minist Educ & Tianjin, Tinajin 300457, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES | 2021年 / 17卷 / 09期

基金：

中国国家自然科学基金;

关键词：

miR-17-5p; MKL-1; gastric cancer; stem cells; bioinformatics; EPITHELIAL-MESENCHYMAL TRANSITION; REGULATES SELF-RENEWAL; ACUTE MYELOID-LEUKEMIA; TRANSCRIPTION FACTOR; MRTF-A; MYOCARDIN; IDENTIFICATION; SUPPRESSION; METASTASIS; INHIBITION;

D O I：

10.7150/ijbs.57338

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Effectively targeting cancer stem cells to treat cancer has great therapeutic prospects. However, the effect of microRNA miR-17/MKL-1 on gastric cancer stem cells has not been studied yet. This study preliminarily explored the mechanism of miR-17/MKL-1 in gastric cancer stem cells. Many previous reports have indicated that microRNA and EMT regulated cancer stem cell characteristics, and miR-17 and MKL-1 were involved as a critical gene in migration and invasion in the EMT pathway. Through RT-PCR, Western Blot, flow cytometry, immunofluorescence, sphere formation xenograft tumor assays and drug resistance, the role of miR-17-5p and MKL-1 on promoting stem cell-like properties of gastric cancer were verified in vivo and vitro. Next, MKL-1 targets CD44, EpCAM, and miR-17-5p promoter verified by luciferase assay and ChIP. Besides, the TCGA database analysis found that both miR-17-5p and MKL-1 increased in gastric cancer, and the prognostic survival of the MKL-1 high expression group was reduced. It is found that MKL-1 promotes expression by targeting miR-17, CD44 and EpCAM promoters. Besides, the TCGA database analysis found that both miR-17-5p and MKL-1 increased in gastric cancer, and the prognostic survival of the MKL-1 high expression group was reduced. These findings reveal new regulatory signaling pathways for gastric cancer stem cells, thus it give new insights on potential early diagnosis and/or molecular therapy for gastric cancer.

引用

页码：2278 / 2293

页数：16

共 56 条

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