In vitro studies on the relative availability and mobility in soil of natural hatching factors for the potato cyst nematodes, Globodera rostochiensis and G-pallida

The fractionation of root leachates from tomato and potato on a Sephadex G-10 column revealed many similarities between the elution profiles of hatching activities towards potato cyst nematodes (PCN). When aliquots of either tomato or potato loot leachate were mixed with different soil samples. hatching factors exhibiting G. pallida-selective hatching activity showed less affinity for the soil matrix than did those selective for G. rostochiensis. This was confirmed by the earlier elution of G. pallida-selective hatching factors from columns in which soil was used as the solid phase in low pressure liquid chromatography. The selectivity of the earliest-eluting hatching factors towards G. pallida, relative to G. rostochiensis, was found to increase significantly as the percentage soil organic matter content increased.