Lentzea cavernae sp nov., an actinobacterium isolated from a karst cave sample, and emended description of the genus Lentzea

A novel actinobacterial strain, designated SYSU K10001(T), was isolated from a limestone sample collected from a karst cave in Xingyi county, Guizhou province, south-western China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were aerobic and Gram-stain-positive. On the basis of 16S rRNA gene sequence analysis, strain SYSU K10001(T) was most closely related to the type strains of the genus Lentzea, Lentzea albida NBRC 16102(T) (98.8% similarity) and Lentzea waywayandensis NRRL B-16159(T) (98.6 %), and is therefore considered to represent a member of the genus Lentzea. DNA-DNA hybridization values between strain SYSU K10001(T) and related type strains of the genus Lentzea were less than 70 %. In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell sugars were arabinose, fructose, mannose and xylose. The major isoprenoid quinone was MK9(H-4), while the major fatty acids (> 10 %) were iso-C-16 : 0 and C-14 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, one unidentified phospholipid and one unidentified lipid. The genomic DNA G+C content of strain SYSU K10001(T) was 69.4 mol%. On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10001(T) represents a novel species of the genus Lentzea, for which the name Lentzea cavernae sp. nov. is proposed. The type strain is SYSU K10001(T) (=KCTC 39804(T) = CGMCC 4.7367(T) = NBRC 112394(T)).