Enrichment of a bipotent hepatic progenitor cell from naive adult liver tissue

被引：20
作者：
Wright, Natasha [1 ]
Samuelson, Lisa [1 ]
Walkup, Maggie H. [1 ]
Chandrasekaran, Prakash [2 ]
Gerber, David A. [1 ]
机构：
[1] Univ N Carolina, Dept Surg, Chapel Hill, NC 27599 USA
[2] Univ N Carolina, Dept Biomed Engn, Chapel Hill, NC 27599 USA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 366卷 / 02期
关键词：
hepatic; liver; progenitor cell; stem cell; bipotential; differentiation;
D O I：
10.1016/j.bbrc.2007.11.129
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Background/Aim: Recent interest in the liver stem cell field has led to the identification and characterization of several hepatic progenitor cell populations from fetal and adult tissues. We isolated a hepatic progenitor cell from naive adult liver and the current studies focus on differentiation and growth. Results: A Sea-1(+) hepatic progenitor cell was identified within the liver parenchyma. This cell expresses numerous liver related genes and transcription found in the developing and/or adult liver. It is located in the peri-portal region and expresses markers associated with undifferentiated hepatic cell populations, mature hepatocytes and biliary cells which distinguish it from the Sca-1(-) fraction. Conclusion: This hepatic progenitor cell from uninjured liver has features of both hepatocytic and biliary populations and demonstrates proliferative potential. Further studies will focus on sca-HPC subsets and conditions that regulate differentiation towards hepatic or biliary lineages. (C) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：367 / 372
页数：6
相关论文
共 31 条
  • [1] Abramoff MD., 2004, Biophot. Int., V11, P36
  • [2] HEMATOPOIETIC STEM-CELL MARKERS ARE EXPRESSED BY DUCTAL PLATE AND BILE-DUCT CELLS IN DEVELOPING HUMAN LIVER
    BLAKOLMER, K
    JASKIEWICZ, K
    DUNSFORD, HA
    ROBSON, SC
    [J]. HEPATOLOGY, 1995, 21 (06) : 1510 - 1516
  • [3] Population expansion, clonal growth, and specific differentiation patterns in primary cultures of hepatocytes induced by HGF/SF, EGF and TGF alpha in a chemically defined (HGM) medium
    Block, GD
    Locker, J
    Bowen, WC
    Petersen, BE
    Katyal, S
    Strom, SC
    Riley, T
    Howard, TA
    Michalopoulos, GK
    [J]. JOURNAL OF CELL BIOLOGY, 1996, 132 (06) : 1133 - 1149
  • [4] Isolation and angiogenesis by endothelial progenitors in the fetal liver
    Cherqui, S
    Kurian, SM
    Schussler, O
    Hewel, JA
    Yates, JR
    Salomon, DR
    [J]. STEM CELLS, 2006, 24 (01) : 44 - 54
  • [5] Choi DH, 2002, CELL TRANSPLANT, V11, P359
  • [6] Liver tissue engineering within alginate scaffolds: Effects of cell-seeding density on hepatocyte viability, morphology, and function
    Dvir-Ginzberg, M
    Gamlieli-Bonshtein, I
    Agbaria, R
    Cohen, S
    [J]. TISSUE ENGINEERING, 2003, 9 (04): : 757 - 766
  • [7] Cell growth and differentiation of different hepatic cells isolated from fetal rat liver in vitro
    Fiegel, HC
    Bruns, H
    Höper, C
    Ltznov, MV
    Kluth, D
    [J]. TISSUE ENGINEERING, 2006, 12 (01): : 123 - 130
  • [8] Plasticity of hepatic cell differentiation:: Bipotential adult mouse liver clonal cell lines competent to differentiate in vitro and in vivo
    Fougere-Deschatrette, Catherine
    Imaizumi-Scherrer, Tereza
    Strick-Marchand, Helene
    Morosan, Serban
    Charneau, Pierre
    Kremsdorf, Dina
    Faust, Daniela M.
    Weiss, Mary C.
    [J]. STEM CELLS, 2006, 24 (09) : 2098 - 2109
  • [9] Purification of adult hepatic progenitor cells using green fluorescent protein (GFP)-transgenic mice and fluorescence-activated cell sorting
    Fujikawa, T
    Hirose, T
    Fujii, H
    Oe, S
    Yasuchika, K
    Azuma, H
    Yamaoka, Y
    [J]. JOURNAL OF HEPATOLOGY, 2003, 39 (02) : 162 - 170
  • [10] FUJIO K, 1994, LAB INVEST, V70, P511
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