Detection of two Bartonella tamiae-like sequences in Amblyomma americanum (Acari: Ixodidae) using 16S-23S intergenic spacer region-specific primers

被引：0

作者：

Billeter, Sarah A. ^{[1
]}

Miller, Melissa K. ^{[2
]}

Breitschwerdt, Edward B. ^{[1
]}

Levy, Michael G. ^{[1
]}

机构：

[1] N Carolina State Univ, Coll Vet Med, Ctr Comparat Med & Transiat Res, Raleigh, NC 27606 USA

[2] USA Ctr, Hlth Promot & Prevent Med N, Ft George G Meade, MD 20755 USA

来源：

JOURNAL OF MEDICAL ENTOMOLOGY | 2008年 / 45卷 / 01期

关键词：

Bartonella; Amblyomma americanum; PCR;

D O I：

10.1603/0022-2585(2008)45[176:DOTBTS]2.0.CO;2

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Four hundred and sixty-six questing Amblyomma americanum (L.) (Acari: Ixodidae) from Carolina County, VA, and 98 questing A. americanum from Chatham County, NC, were screened by polymerase chain reaction (PCR) for the Bartonella 16S-23S intergenic spacer region. Two amplicons, approximate to 270-280 bp, were detected in two ticks from Virginia. Based upon PCR and sequencing, an adult male and adult female tick harbored DNA sequences closely related to Bartonella tamiae (DQ395180). Bartonella DNA was not detected in A. americanum from North Carolina. Potential transmission of Bartonella spp. by A. arnericanuin should be the focus of future experimental studies.

引用

页码：176 / 179

页数：4

共 6 条

[1] MOLECULAR DETECTION OF BARTONELLA SP IN WILD RUMINANTS AND ANALYSIS OF ITS GENETIC DIVERSITY ON THE BASIS OF 16S-23S RRNA INTERGENIC SPACER (ITS)
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[2] Two Genotypes among 'Candidatus Mycoplasma haemobos' Strains Based on the 16S-23S rRNA Intergenic Spacer Sequences
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[3] Identification of Bartonella strains isolated from wild and domestic ruminants by a single-step PCR analysis of the 16S-23S intergenic spacer region
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[4] Identification of oligonucleotide primers targeted at the 16S-23S rDNA intergenic spacers for genus- and species-specific detection of aeromonads
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[5] Rapid and direct detection of Clostridium chauvoei by PCR of the 16S-23S rDNA spacer region and partial 23S rDNA sequences
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[6] Sequencing of an internal transcribed spacer region of 16S-23S rRNA gene and designing of PCR primers for the detection of Salmonella spp. in food
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