Heterologous production of flavanones in Escherichia coli:: potential for combinatorial biosynthesis of flavonoids in bacteria

被引:63
作者
Kaneko, M [1 ]
Hwang, EI [1 ]
Ohnishi, Y [1 ]
Horinouchi, S [1 ]
机构
[1] Univ Tokyo, Dept Biotechnol, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
关键词
combinatorial biosynthesis; flavonoid flavanone; chalcone; metabolic engineering; 4-coumarate/cinnamate : CoA ligase;
D O I
10.1007/s10295-003-0061-1
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Chalcones, the central precursor of flavonoids, are synthesized exclusively in plants from tyrosine and phenylalanine via the sequential reaction of phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H), 4-coumarate: coenzyme A ligase (4CL) and chalcone synthase (CHS). Chalcones are converted into the corresponding flavanones by the action of chalcone isomerase (CHI), or non-enzymatically under alkaline conditions. PAL from the yeast Rhodotorula rubra, 4CL from an actinomycete Streptomyces coelicolor A3(2), and CHS from a licorice plant Glycyrrhiza echinata, assembled as artificial gene clusters in different organizations, were used for fermentation production of flavanones in Escherichia coli. Because the bacterial 4CL enzyme attaches CoA to both cinnamic acid and 4-coumaric acid, the designed biosynthetic pathway bypassed the C4H step. E. coli carrying one of the designed gene clusters produced about 450 mug naringenin/l from tyrosine and 750 mug pinocembrin/l from phenylalanine. The successful production of plant-specific flavanones in bacteria demonstrates the usefulness of combinatorial biosynthesis approaches not only for the production of various compounds of plant and animal origin but also for the construction of libraries of "unnatural" natural compounds.
引用
收藏
页码:456 / 461
页数:6
相关论文
共 50 条
[31]   Engineering Escherichia coli towards de novo production of gatekeeper (2S)-flavanones: naringenin, pinocembrin, eriodictyol and homoeriodictyol [J].
Dunstan, Mark S. ;
Robinson, Christopher J. ;
Jervis, Adrian J. ;
Yan, Cunyu ;
Carbonell, Pablo ;
Hollywood, Katherine A. ;
Currin, Andrew ;
Swainston, Neil ;
Le Feuvre, Rosalind ;
Micklefield, Jason ;
Faulon, Jean-Loup ;
Breitling, Rainer ;
Turner, Nicholas ;
Takano, Eriko ;
Scrutton, Nigel S. .
SYNTHETIC BIOLOGY, 2020, 5 (01)
[32]   Combinatorial biosynthesis of Synechocystis PCC6803 phycocyanin holo-α-subunit (CpcA) in Escherichia coli and its activities [J].
Yu, Ping ;
Li, Peng ;
Chen, Xingge ;
Chao, Xiaoyin .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 100 (12) :5375-5388
[33]   Combinatorial biosynthesis of Synechocystis PCC6803 phycocyanin holo-α-subunit (CpcA) in Escherichia coli and its activities [J].
Ping Yu ;
Peng Li ;
Xingge Chen ;
Xiaoyin Chao .
Applied Microbiology and Biotechnology, 2016, 100 :5375-5388
[34]   Combinatorial metabolic engineering of Escherichia coli for de novo production of structurally defined and homogeneous Amino oligosaccharides [J].
Shi, Jinqi ;
Deng, Chen ;
Zhang, Chunyue ;
Quan, Shu ;
Fan, Liqiang ;
Zhao, Liming .
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY, 2024, 9 (04) :713-722
[35]   Simultaneous production and partitioning of heterologous polyketide and isoprenoid natural products in an Escherichia coli two-phase bioprocess [J].
Boghigian, Brett A. ;
Myint, Melissa ;
Wu, Jiequn ;
Pfeifer, Blaine A. .
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2011, 38 (11) :1809-1820
[36]   Improved production of 3-hydroxypropionic acid in engineered Escherichia coli by rebalancing heterologous and endogenous synthetic pathways [J].
Lee, Tae-Young ;
Min, Won-Ki ;
Kim, Hyo Jin ;
Seo, Jin-Ho .
BIORESOURCE TECHNOLOGY, 2020, 299
[37]   Increasing the metabolic capacity of Escherichia coli for hydrogen production through heterologous expression of the Ralstonia eutropha SH operon [J].
Dipankar Ghosh ;
Ariane Bisaillon ;
Patrick C Hallenbeck .
Biotechnology for Biofuels, 6
[38]   Increasing the metabolic capacity of Escherichia coli for hydrogen production through heterologous expression of the Ralstonia eutropha SH operon [J].
Ghosh, Dipankar ;
Bisaillon, Ariane ;
Hallenbeck, Patrick C. .
BIOTECHNOLOGY FOR BIOFUELS, 2013, 6
[39]   Increased Malonyl Coenzyme A Biosynthesis by Tuning the Escherichia coli Metabolic Network and Its Application to Flavanone Production [J].
Fowler, Zachary L. ;
Gikandi, William W. ;
Koffas, Mattheos A. G. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009, 75 (18) :5831-5839
[40]   Investigating the Role of Native Propionyl-CoA and Methylmalonyl-CoA Metabolism on Heterologous Polyketide Production in Escherichia coli [J].
Zhang, Haoran ;
Boghigian, Brett A. ;
Pfeifer, Blaine A. .
BIOTECHNOLOGY AND BIOENGINEERING, 2010, 105 (03) :567-573