Phylogenetic analysis of recent classical swine fever virus (CSFV) isolates from Assam, India

被引:39
作者
Sarma, Dilip Kumar [2 ]
Mishra, Niranjan [1 ]
Vilcek, Stefan [3 ]
Rajukumar, Katherukamem [1 ]
Behera, Sthita Pragnya [1 ]
Nema, Ram Kumar [1 ]
Dubey, Pooja [1 ]
Dubey, Shiv Chandra [1 ]
机构
[1] Indian Vet Res Inst, High Secur Anim Dis Lab, Bhopal 462021, Madhya Pradesh, India
[2] Assam Agr Univ, Gauhati 781022, Assam, India
[3] Univ Vet Med, Dept Infect Dis & Epizootiol, Kosice, Slovakia
关键词
Classical swine fever virus; E2; India; NS5B; Phylogenetic analysis; 5 ' UTR; OUTBREAKS; DATABASE; PIGS; E2;
D O I
10.1016/j.cimid.2009.09.005
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Classical swine fever (CSF), a highly contagious viral disease of pigs, is endemic in India. As there is no information concerning the accurate genetic typing of classical swine fever virus (CSFV) isolates in India, 16 CSF viruses isolated during 2005-2007 from domestic pigs in different districts of Assam were typed in 5' UTR (150 nucleotides). To confirm the genetic typing results and to study the genetic variability, selected viruses were also analyzed in E2 (190 nt) and NS5B gene (409 nt) regions. Phylogenetic analysis revealed that all the 16 CSFV isolates analyzed belonged to group 1 and subgroup 1.1 in contrast to the situation in other Asian countries. Additionally, analysis in E2 and NS5B region placed the Indian isolates in a clearly separated clade within subgroup 1.1. The results suggest that subgroup 1.1 CSF viruses are currently circulating in India, which is important for epidemiology and control of CSF. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:11 / 15
页数:5
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