Identification, tissue distribution and orexigenic activity of neuropeptide F (NPF) in penaeid shrimp

被引:48
作者
Christie, Andrew E. [1 ]
Chapline, M. Christine [1 ]
Jackson, James M. [2 ]
Dowda, Jenilee K. [3 ]
Hartline, Niko [2 ]
Malecha, Spencer R. [3 ]
Lenz, Petra H. [2 ]
机构
[1] John W & Jean C Boylan Ctr Cellular & Mol Physiol, Neurosci Program, Mt Desert Isl Biol Lab, Salsbury Cove, ME 04672 USA
[2] Univ Hawaii Manoa, Pacific Biosci Res Ctr, Bekesy Lab Neurobiol, Honolulu, HI 96822 USA
[3] Univ Hawaii Manoa, Dept Human Nutr Food & Anim Sci, Coll Trop Agr & Human Resources, Honolulu, HI 96822 USA
基金
美国国家科学基金会;
关键词
Litopenaeus vannamei; Marsupenaeus japonicus; Melicertus marginatus; FMRFamide-like peptide (FLP); neuropeptide F; neuropeptide Y; TACHYKININ-RELATED PEPTIDE; IN-SILICO ANALYSES; ENDOCRINE-CELLS; AMERICAN LOBSTER; NERVOUS-SYSTEM; HYPERGLYCEMIC HORMONE; Y HOMOLOG; EVOLUTION; EXPRESSION; GUT;
D O I
10.1242/jeb.053173
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The neuropeptide Fs (NPFs) are an invertebrate subgroup of the FMRFamide-like peptides, and are proposed by some to be the homologs of vertebrate neuropeptide Y. Although there is some information about the identity, tissue distribution and function of NPFs in insects, essentially nothing is known about them in crustaceans. We have identified and characterized NPF-encoding transcripts from the penaeid shrimp Litopenaeus vannamei and Melicertus marginatus. Two transcripts were identified from each species. For each shrimp species, the two transcripts differed from one another by the presence or absence of an insert in the portion of the open reading frame that encodes the NPF peptide. The two NPF isoforms are identical in L. vannamei and M. marginatus, with their predicted structures being KPDPSQLANMAEALKYLQELDKYYSQVSRPRFamide and KPDPSQLANMAEALKYLQELDKYYSQVSRPSPRSAPGPASQIQALENTLKFLQLQELGKLYSLRARPRFamide. RT-PCR tissue profiling showed both transcripts are broadly distributed within the nervous system of each species. The transcript encoding the shorter NPF was detected in some, but not all, midgut samples. The transcript encoding the longer NPF was absent in the midgut of both species, and neither transcript was detected in their skeletal muscle. Juvenile L. vannamei fed on a diet supplemented with the shorter NPF exhibited a marked increase in food intake relative to control individuals that did not receive the supplement; the NPF-fed shrimp also showed a significant increase in growth relative to the control group. Our data suggest that NPF is present in both the nervous system and midgut of penaeid shrimp, functioning, at least in part, as a powerful orexigenic agent.
引用
收藏
页码:1386 / 1396
页数:11
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