High resolution imaging of surface patterns of single bacterial cells

被引：36

作者：

Greif, Dominik ^{[1
]}

Wesner, Daniel ^{[1
]}

Regtmeier, Jan ^{[1
]}

Anselmetti, Dario ^{[1
]}

机构：

[1] Univ Bielefeld, D-33615 Bielefeld, Germany

来源：

ULTRAMICROSCOPY | 2010年 / 110卷 / 10期

关键词：

AFM; SEM; Artefact; Cell surface; Life cell imaging; Sample preparation; ATOMIC-FORCE MICROSCOPY; ESCHERICHIA-COLI; SINORHIZOBIUM-MELILOTI; MEMBRANE-VESICLES; OUTER-MEMBRANE; PROTEINS; ALFALFA; MUTANT; SYMBIOSIS; PLANTS;

D O I：

10.1016/j.ultramic.2010.06.004

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

We systematically studied the origin of surface patterns observed on single Sinorhizobium meliloti bacterial cells by comparing the complementary techniques atomic force microscopy (AFM) and scanning electron microscopy (SEM). Conditions ranged from living bacteria in liquid to fixed bacteria in high vacuum. Stepwise, we applied different sample modifications (fixation, drying, metal coating, etc.) and characterized the observed surface patterns. A detailed analysis revealed that the surface structure with wrinkled protrusions in SEM images were not generated de novo but most likely evolved from similar and naturally present structures on the surface of living bacteria. The influence of osmotic stress to the surface structure of living cells was evaluated and also the contribution of exopolysaccharide and lipopolysaccharide (LPS) by imaging two mutant strains of the bacterium under native conditions. AFM images of living bacteria in culture medium exhibited surface structures of the size of single proteins emphasizing the usefulness of AFM for high resolution cell imaging. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：1290 / 1296

页数：7

共 29 条

[1] High-resolution atomic force microscopy studies of the Escherichia coli outer membrane:: Structural basis for permeability [J].