Acceptor specificity of cellobiose phosphorylase from Cellvibrio gilvus:: synthesis of three branched trisaccharides

被引：35

作者：

Percy, A ^{[1
]}

Ono, H ^{[1
]}

Hayashi, K ^{[1
]}

机构：

[1] Natl Food Res Inst, Ibaraki, Osaka 305, Japan

来源：

CARBOHYDRATE RESEARCH | 1998年 / 308卷 / 3-4期

关键词：

beta-D-glucopyranosyl-(1 -> 4)-D-glucuronamide; beta-D-glucopyranosyl-(1 -> 4)-[alpha-D-galactopyranosyl-(1 -> 6)]-D-glucopyranose; beta-D-glucopyranosyl-(1 -> 4)-[beta-D-glucopyranosyl-(1 -> 6)]-D-glucopyranose; beta-D-glucopyranosyl-(1 -> 4)-[alpha-D-glucopyranosyl-(1 -> 6)]-D-glucopyranose; Cellvibrio gilvus; cellobiose phosphorylase;

D O I：

10.1016/S0008-6215(98)00109-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cellobiose phosphorylase from Cellvibrio gilvus was examined for its acceptor specificity in the synthetic reaction with glucose-1-phosphate, using substrates in which the C-6 substituent of D-Glc had been altered. A range of disaccharides were also tested for acceptor specificity but only those with (1-->6)-linkages were successful accepters. Melibiose, gentiobiose, isomaltose and also the monosaccharide glucuronamide were found to react with cellobiose phosphorylase and glucose-1-phosphate giving beta-D-Glcp-(1-->4)-[alpha-D-Galp-(1-->6)]-D-Glcp, beta-D-Glcp-(1-->4)-[beta-D-Glcp-(1-->6)]-D-Glcp, beta-D-Glcp-(1-->4)-[alpha-D-Glcp-(1-->6)]-D-Glcp and beta-D-Glcp-(1-->4)-D-GlcUNp, respectively. These products were purified using a range of chromatographic methods and characterised by NMR and FAR-MS. This is the first time cellobiose phosphorylase has been shown to synthesise trisaccharides. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：423 / 429

页数：7

共 14 条

[1] SYNTHESIS OF 4-O-BETA-D-GLUCOPYRANOSYL-D-XYLOSE 4-O-BETA-D-GLUCOPYRANOSYL-D-ARABINOSE 4-O-BETA-D-GLUCOPYRANOSYL-2-DEOXY-D-GLUCOSE 4-O-BETA-D-GLUCOPYRANOSYL-D-MANNOSE AND 4-O-BETA-D-GLUCOPYRANOSYL-D-GLUCOSAMINE BY CELLOBIOSE PHOSPHORYLASE FROM CLOSTRIDIUM THERMOCELLUM [J].