Evaluation of characteristics and in vitro antioxidant properties of RSV loaded hyaluronic acid-DPPC microparticles as a wound healing system

被引：49

作者：

Eroglu, Ipek ^{[1
]}

Gokce, Evren H. ^{[1
]}

Tsapis, Nicolas ^{[2
]}

Tanriverdi, Sakine Tuncay ^{[1
]}

Gokce, Goksel ^{[3
]}

Fattal, Elias ^{[2
]}

Ozer, Ozgen ^{[1
]}

机构：

[1] Ege Univ, Fac Pharm, Dept Pharmaceut Technol, TR-35100 Izmir, Turkey

[2] Univ Paris 11, Fac Pharm, Inst Gallen Paris Sud, UMR CNRS 8612, F-92290 Chatenay Malabry, France

[3] Univ Ege, Fac Pharm, Dept Pharmacol, TR-35100 Izmir, Turkey

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2015年 / 126卷

关键词：

Microparticles; Resveratrol; Hyaluronic acid; Wound healing; Antioxidant; SUPRAMOLECULAR ORGANIZATION; DEGRADATION; FORMULATION; MECHANISMS; VIVO;

D O I：

10.1016/j.colsurfb.2014.12.006

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Resveratrol (RSV) was incorporated into microparticles by spray drying to treat chronic wounds such as diabetic ulcers. RSV was chosen due to its defense mechanisms as the formation of free radicals delays the healing process. RSV was loaded into microparticles consisting of dipalmitoylphosphatidylcholine (DPPC) and hyaluronic acid (HA), a polysaccharide naturally present within the skin, known to contribute to the healing process. Microparticles were evaluated in terms of production yield, size distribution, encapsulation efficiency, morphology, specific surface area, thermal properties and water content. Spherical and homogenous microparticles (span <= 2) in a size range between 20 and 30 mu m were obtained with high encapsulation efficiency (>= 97%). The effect of enzymes (hyaluronidase, phospholipase and lipase) on RSV release showed a dose-dependent pattern followed by a slow release stage. Cytotoxicity/proliferation and oxidative stress parameters (glutathione, oxidized glutathione, glutathione peroxidase, malondialdehyde, superoxide dismutase) obtained from human dermal fibroblast cell cultures revealed that formulations increased cell proliferation and the presence of RSV decreased oxidation in cells. RSV-loaded HA-DPPC microparticles appear as a promising formulation for wound healing due to synergistic effect of the ingredients. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：50 / 57

页数：8

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