Determination of phospholipid and fatty glyceride in liposome by RP-HPLC with capacitively coupled contactless conductivity detector

被引：4

作者：

Huang, Xing ^{[1
]}

Zhang, Kaipeng ^{[1
]}

Tong, Yanli ^{[2
]}

Zhong, Yongsheng ^{[3
]}

Chen, Zuanguang ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China

[2] Guangdong Second Prov Gen Hosp, Guangzhou 510310, Guangdong, Peoples R China

[3] Sun Yat Sen Univ, East Campus Lab Ctr, Guangzhou 510006, Guangdong, Peoples R China

来源：

ANALYTICAL METHODS | 2018年 / 10卷 / 41期

基金：

中国国家自然科学基金;

关键词：

PERFORMANCE LIQUID-CHROMATOGRAPHY; CAPILLARY-ELECTROPHORESIS; ION CHROMATOGRAPHY; DETECTION CE-(CD)-D-4; FUNDAMENTAL-ASPECTS; PEPTIDES; COLUMN;

D O I：

10.1039/c8ay01635f

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this study, a gradient reverse phase high performance liquid chromatography combined with a capacitively coupled contactless conductivity detection (RP-HPLC-(CD)-D-4) method for simultaneous determination of phospholipid and fatty glyceride in liposome was developed. After saponification, the fatty glyceride and two types of phospholipids were transformed into octanoic acid, palmitic acid and erucic acid, respectively, which were then separated and detected by RP-HPLC-(CD)-D-4. The results showed good linearity in the range of 20-1000 mu g mL(-1) for octanoic acid and erucic acid and 10-1000 mu g mL(-1) for palmitic acid. The LODs of octanoic acid and palmitic acid reached 2.5 mu g mL(-1), and 10 mu g mL(-1) for erucic acid. The average recoveries of octanoic acid, palmitic acid and erucic acid were 100.2%, 102.9% and 101.4% (n = 9), respectively. The developed method could be effectively applied in the quality control and stability monitoring of liposome.

引用

页码：4978 / 4984

页数：7

共 30 条

[1] CATION PERMEABILITY OF PHOSPHOLIPID MODEL MEMBRANES - EFFECT OF NARCOTICS
BANGHAM, AD
STANDISH, MM
MILLER, N
[J]. NATURE, 1965, 208 (5017) : 1295 - +
[2] LC-MS analysis of phospholipids and lysophospholipids in human bronchoalveolar lavage fluid
Barroso, B
Bischoff, R
[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2005, 814 (01): : 21 - 28
[3] da Silva JAF, 1998, ANAL CHEM, V70, P4339
[4] Recent applications and developments of capacitively coupled contactless conductivity detection (CE-C4D) in capillary electrophoresis
Elbashir, Abdalla A.
Aboul-Enein, Hassan Y.
[J]. BIOMEDICAL CHROMATOGRAPHY, 2014, 28 (11) : 1502 - 1506
[5] Applications of capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D) in pharmaceutical and biological analysis
Elbashir, Abdalla A.
Aboul-Enein, Hassan Y.
[J]. BIOMEDICAL CHROMATOGRAPHY, 2010, 24 (10) : 1038 - 1044
[6] Use of contactless conductivity detection for non-invasive characterisation of monolithic stationary-phase coatings for application in capillary ion chromatography
Gillespie, Eoin
Connolly, Damian
Macka, Miroslav
Nesterenko, Pavel N.
Paull, Brett
[J]. ANALYST, 2007, 132 (12) : 1238 - 1245
[7] Evaluation of capillary ion exchange stationary phase coating distribution and stability using radial capillary column contactless conductivity detection
Gillespie, Eoin
Macka, Miroslav
Connolly, Damien
Paull, Brett
[J]. ANALYST, 2006, 131 (08) : 886 - 888
[8] Hamplová A, 2008, CHEM LISTY, V102, P194
[9] Evaluation of contactless conductivity detection for the determination of UV absorbing and non-UV absorbing species in reversed-phase high-performance liquid chromatography
Kubán, P
Abad-Villar, EM
Hauser, PC
[J]. JOURNAL OF CHROMATOGRAPHY A, 2006, 1107 (1-2) : 159 - 164
[10] Fundamental aspects of contactless conductivity detection for capillary electrophoresis. Part I: Frequency behavior and cell geometry
Kuban, P
Hauser, PC
[J]. ELECTROPHORESIS, 2004, 25 (20) : 3387 - 3397

← 1 2 3 →