Highly efficient siRNA delivery system into human and murine cells using single-wall carbon nanotubes

被引：67

作者：

Ladeira, M. S. ^{[1
]}

Andrade, V. A. ^{[1
]}

Gomes, E. R. M. ^{[1
]}

Aguiar, C. J. ^{[1
]}

Moraes, E. R. ^{[1
]}

Soares, J. S. ^{[2
]}

Silva, E. E. ^{[2
]}

Lacerda, R. G. ^{[2
]}

Ladeira, L. O. ^{[2
]}

Jorio, A. ^{[2
]}

Lima, P. ^{[3
]}

Leite, M. Fatima ^{[1
,4
]}

Resende, R. R. ^{[2
,5
]}

Guatimosim, S. ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Dept Physiol & Biophys, BR-31270901 Belo Horizonte, MG, Brazil

[2] Univ Fed Minas Gerais, Dept Phys, BR-31270901 Belo Horizonte, MG, Brazil

[3] Univ Fed Sao Joao del Rei, Dept Biosyst Engn, BR-36307352 Sao Joao Del Rei, MG, Brazil

[4] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA

[5] Univ Fed Sao Joao del Rei, Dept Biochem, Adv Ctr Hlth, BR-35501296 Divinopolis, MG, Brazil

来源：

NANOTECHNOLOGY | 2010年 / 21卷 / 38期

关键词：

INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR; NEAR-INFRARED FLUORESCENCE; SMALL INTERFERING RNA; INTRACELLULAR DELIVERY; MAMMALIAN-CELLS; IN-VITRO; CALCIUM; FUNCTIONALIZATION; RETICULUM; SIGNALS;

D O I：

10.1088/0957-4484/21/38/385101

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Development of RNA interference (RNAi) technology utilizing short interfering RNA sequences (siRNA) has focused on creating methods for delivering siRNAs to cells and for enhancing siRNA stability in vitro and in vivo. Here, we describe a novel approach for siRNA cellular delivery using siRNA coiling into carboxyl-functionalized single-wall carbon nanotubes (SWCNTs). The CNT-siRNA delivery system successfully demonstrates nonspecific toxicity and transfection efficiency greater than 95%. This approach offers the potential for siRNA delivery into different types of cells, including hard-to-transfect cells, such as neuronal cells and cardiomyocytes. We also tested the CNT-siRNA system in a non-metastatic human hepatocellular carcinoma cell line (SKHep1). In all types of cells used in this work the CNT-siRNA delivery system showed high efficiency and apparent no side effects for various in vitro applications.

引用

页数：12

共 32 条

[1] Indoor allergen sensitization profile in allergic patients of the allergy clinic in the University Hospital in Uberlandia, Brazil [J].

Almeida Scares, Fernanda Aparecida ;

Silva Segundo, Gesmar Rodrigues ;

Alves, Ronaldo ;

Ynoue, Leandro Hideki ;

Resende, Rafael Oliveira ;

Sopelete, Monica Camargo ;

Oliveira Silva, Dose Aparecida ;

Sung, Sun-Sang Joseph ;

Taketomi, Ernesto A. .

REVISTA DA ASSOCIACAO MEDICA BRASILEIRA, 2007, 53 (01) :25-28

[2]

Blackmore J., 2000, INT J LEADERSH EDUC, V3, P1, DOI DOI 10.1080/136031200292830

[3] Highly efficient molecular delivery into mammalian cells using carbon nanotube spearing [J].

Cai, D ;

Mataraza, JM ;

Qin, ZH ;

Huang, ZP ;

Huang, JY ;

Chiles, TC ;

Carnahan, D ;

Kempa, K ;

Ren, ZF .

NATURE METHODS, 2005, 2 (06) :449-454

[4] Notch activates cell cycle reentry and progression in quiescent cardiomyocytes [J].

Campa, Victor M. ;

Gutierrez-Lanza, Raquel ;

Cerignoli, Fabio ;

Diaz-Trelles, Ramon ;

Nelson, Brandon ;

Tsuji, Toshiya ;

Barcova, Maria ;

Jiang, Wei ;

Mercola, Mark .

JOURNAL OF CELL BIOLOGY, 2008, 183 (01) :129-141

[5] Near-infrared fluorescence microscopy of single-walled carbon nanotubes in phagocytic cells [J].

Cherukuri, P ;

Bachilo, SM ;

Litovsky, SH ;

Weisman, RB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (48) :15638-15639

[6] Mammalian pharmacokinetics of carbon nanotubes using intrinsic near-infrared fluorescence [J].

Cherukuri, Paul ;

Gannon, Christopher J. ;

Leeuw, Tonya K. ;

Schmidt, Howard K. ;

Smalley, Richard E. ;

Curley, Steven A. ;

Weisman, R. Bruce .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (50) :18882-18886

[7] Effects of antisense-myc-conjugated single-walled carbon nanotubes on HL-60 cells [J].

Cui, Daxiang ;

Tian, Furong ;

Coyer, Sean R. ;

Wang, Jicun ;

Pan, Bifeng ;

Gao, Feng ;

He, Rong ;

Zhang, Yafei .

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2007, 7 (4-5) :1639-1646

[8] Nanostructured 3-D Collagen/Nanotube Biocomposites for Future Bone Regeneration Scaffolds [J].

da Silva, Edelma E. ;

Della Colleta, Heloisa H. M. ;

Ferlauto, Andre S. ;

Moreira, Roberto L. ;

Resende, Rodrigo R. ;

Oliveira, Sergio ;

Kitten, Gregory T. ;

Lacerda, Rodrigo G. ;

Ladeira, Luiz O. .

NANO RESEARCH, 2009, 2 (06) :462-473

[9]

Dent MAR, 1996, DEVELOPMENT, V122, P1029

[10] Carbon nanotube fibers are compatible with mammalian cells and neurons [J].

Dubin, Robert A. ;

Callegari, Gerardo C. ;

Kohn, Joachim ;

Neimark, Alexander V. .

IEEE TRANSACTIONS ON NANOBIOSCIENCE, 2008, 7 (01) :11-14

← 1 2 3 4 →