Interplay of Geometric Cues and RGD/BMP-2 Crosstalk in Directing Stem Cell Fate

被引：22

作者：

Bilem, Ibrahim ^{[1
,2
,3
,4
,5
]}

Chevallier, Pascale ^{[1
,2
]}

Plawinski, Laurent ^{[3
,4
,5
]}

Sone, Eli D. ^{[6
,7
]}

Durrieu, Marie-Christine ^{[3
,4
,5
]}

Laroche, Gaetan ^{[1
,2
]}

机构：

[1] Univ Laval, Dept Genie Mines Met & Mat, Ctr Rech Mat Avances, Lab Ingn Surface, 1065 Ave Med, Quebec City, PQ 1065, Canada

[2] Ctr Hosp Univ Quebec, Ctr Rech, Hop St Francois Assise, 10 Rue Espinay, Quebec City, PQ G1L 3L5, Canada

[3] Univ Bordeaux, CBMN, UMR 5248, F-33600 Pessac, France

[4] CNRS, Inst Chem & Biol Membranes & Nanoobjects CBMN 524, F-33600 Pessac, France

[5] Bordeaux INP, UMR 5248, CBMN, F-33600 Pessac, France

[6] Univ Toronto, Inst Biomat & Biomed Engn, Dept Mat Sci & Engn, Toronto, ON M5S 3G9, Canada

[7] Univ Toronto, Fac Dent, Toronto, ON M5S 3G9, Canada

来源：

ACS BIOMATERIALS SCIENCE & ENGINEERING | 2017年 / 3卷 / 10期

基金：

加拿大自然科学与工程研究理事会;

关键词：

chemical micropatterning; BMP-2 mimetic peptide; adhesive ligands; RGD/BMP-2; crosstalk; stem cell niche; bone tissue engineering; EXTRACELLULAR-MATRIX; MICROPATTERNED SURFACES; GROWTH-FACTORS; DIFFERENTIATION; BMP-2; INTEGRIN; ADHESION; KINASE; RGD; ACTIVATION;

D O I：

10.1021/acsbiomaterials.7b00279

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Within the native microenvironment, extracellular matrix (ECM) components are thought to display a complex and heterogeneous distribution, spanning several length scales. Herein, the objective is to mimic, in vitro, the hierarchical organization of proteins and growth factors as well as their crosstalk. Photolithography technique was used to adjacently pattern geometrically defined regions of RGD and BMP-2 mimetic peptides onto glass substrates. These ECM-derived ligands are known to jointly regulate mesenchymal stem cells (MSCs) osteogenic differentiation. By manipulating the spatial distribution of dually grafted peptides, the extent of human MSCs osteogenic differentiation was significantly affected, depending on the shape of peptide micropatterns. Our data highlight the existence of a strong interplay between geometric cues and biochemical signals. Such in vitro systems provide a valuable tool to investigate mechanisms by which multiple ECM cues overlap to regulate stem cell fate, thereby contributing to the design of bioinspired biomaterials for bone tissue engineering applications.

引用

页码：2514 / 2523

页数：10

共 58 条

[1] Physical, Spatial, and Molecular Aspects of Extracellular Matrix of In Vivo Niches and Artificial Scaffolds Relevant to Stem Cells Research [J].