Identification of immunoreactive secretory proteins from the stationary phase culture of Burkholderia pseudomallei

被引：15

作者：

Vellasamy, Kumutha Malar ^{[1
]}

Mariappan, Vanitha ^{[1
]}

Hashim, Onn H. ^{[2
,3
]}

Vadivelu, Jamuna ^{[1
]}

机构：

[1] Univ Malaya, Fac Med, Dept Med Microbiol, Kuala Lumpur 50603, Malaysia

[2] Univ Malaya, Fac Med, Dept Mol Med, Kuala Lumpur 50603, Malaysia

[3] Univ Malaya, Ctr Prote Res, Kuala Lumpur 50603, Malaysia

来源：

ELECTROPHORESIS | 2011年 / 32卷 / 02期

关键词：

Burkholderia pseudomallei; Immunoreactive; Proteomics; Secreted proteins; GROUP-A STREPTOCOCCI; PYRUVATE-DEHYDROGENASE; PROTEOME ANALYSIS; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; MYCOBACTERIUM-TUBERCULOSIS; BACILLUS-SUBTILIS; SURFACE; CELLS; MELIOIDOSIS; ACTIVATION;

D O I：

10.1002/elps.201000355

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacterial secreted proteins are known to be involved in virulence and may mediate important host-pathogen interactions. In this study, when the stationary phase culture supernatant of Burkholderia pseudomallei was subjected to 2-DE, 113 protein spots were detected. Fifty-four of the secreted proteins, which included metabolic enzymes, transcription/translation regulators, potential virulence factors, chaperones, transport regulators, and hypothetical proteins, were identified using MS and database search. Twelve of these proteins were apparently reactive to antisera of mice that were immunised with B. pseudomallei secreted proteins. These proteins might be excellent candidates to be used as diagnostic markers or putative candidate vaccines against B. pseudomallei infections.

引用

页码：310 / 320

页数：11

共 59 条

[31] Identification of immunogenic proteins from Burkholderia cepacia secretome using proteomic analysis
Mariappan, Vanitha
Vellasamy, Kumutha Malar
Thimma, Jaikumar Subramanian
Hashim, Onn H.
Vadivelu, Jamuna
[J]. VACCINE, 2010, 28 (05) : 1318 - 1324
[32] Yersinia-hsp60-reactive T cells are efficiently stimulated by peptides of 12 and 13 amino acid residues in a MHC class II (I-A(b))-restricted manner
Noll, A
Autenrieth, IB
[J]. CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 1996, 105 (02) : 231 - 237
[33] Integrative genomic, transcriptional, and proteomic diversity in natural isolates of the human pathogen Burkholderia pseudomallei
Ou, KL
Ong, C
Koh, SY
Rodrigues, F
Sim, SH
Wong, D
Ooi, CH
Ng, KC
Jikuya, H
Yau, CC
Soon, SY
Kesuma, D
Lee, MA
Tan, P
[J]. JOURNAL OF BACTERIOLOGY, 2005, 187 (12) : 4276 - 4285
[34] Genomic analysis of secretion systems
Pallen, MJ
Chaudhuri, RR
Henderson, IR
[J]. CURRENT OPINION IN MICROBIOLOGY, 2003, 6 (05) : 519 - 527
[35] GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE ON THE SURFACE OF GROUP-A STREPTOCOCCI IS ALSO AN ADP-RIBOSYLATING ENZYME
PANCHOLI, V
FISCHETTI, VA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) : 8154 - 8158
[36] A MAJOR SURFACE PROTEIN ON GROUP-A STREPTOCOCCI IS A GLYCERALDEHYDE-3-PHOSPHATE-DEHYDROGENASE WITH MULTIPLE BINDING-ACTIVITY
PANCHOLI, V
FISCHETTI, VA
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1992, 176 (02) : 415 - 426
[37] Altered secretome of Burkholderia pseudomallei induced by salt stress
Pumirat, Pornpan
Saetun, Putita
Sinchaikul, Supachok
Chen, Shui-Tein
Korbsrisate, Sunee
Thongboonkerd, Visith
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2009, 1794 (06): : 898 - 904
[38] Towards the proteome of Burkholderia cenocepacia H111:: Setting up a 2-DE reference map
Riedel, K
Carranza, P
Gehrig, P
Potthast, F
Eberl, L
[J]. PROTEOMICS, 2006, 6 (01) : 207 - 216
[39] Public health assessment of potential biological terrorism agents
Rotz, LD
Khan, AS
Lillibridge, SR
Ostroff, SM
Hughes, JM
[J]. EMERGING INFECTIOUS DISEASES, 2002, 8 (02) : 225 - 230
[40] Identification of novel proteins secreted by Lactobacillus rhamnosus GG grown in de Mann-Rogosa-Sharpe broth
Sanchez, B.
Schmitter, J. -M.
Urdaci, M. C.
[J]. LETTERS IN APPLIED MICROBIOLOGY, 2009, 48 (05) : 618 - 622

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