Identification of immunoreactive secretory proteins from the stationary phase culture of Burkholderia pseudomallei

被引：15

作者：

Vellasamy, Kumutha Malar ^{[1
]}

Mariappan, Vanitha ^{[1
]}

Hashim, Onn H. ^{[2
,3
]}

Vadivelu, Jamuna ^{[1
]}

机构：

[1] Univ Malaya, Fac Med, Dept Med Microbiol, Kuala Lumpur 50603, Malaysia

[2] Univ Malaya, Fac Med, Dept Mol Med, Kuala Lumpur 50603, Malaysia

[3] Univ Malaya, Ctr Prote Res, Kuala Lumpur 50603, Malaysia

来源：

ELECTROPHORESIS | 2011年 / 32卷 / 02期

关键词：

Burkholderia pseudomallei; Immunoreactive; Proteomics; Secreted proteins; GROUP-A STREPTOCOCCI; PYRUVATE-DEHYDROGENASE; PROTEOME ANALYSIS; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; MYCOBACTERIUM-TUBERCULOSIS; BACILLUS-SUBTILIS; SURFACE; CELLS; MELIOIDOSIS; ACTIVATION;

D O I：

10.1002/elps.201000355

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacterial secreted proteins are known to be involved in virulence and may mediate important host-pathogen interactions. In this study, when the stationary phase culture supernatant of Burkholderia pseudomallei was subjected to 2-DE, 113 protein spots were detected. Fifty-four of the secreted proteins, which included metabolic enzymes, transcription/translation regulators, potential virulence factors, chaperones, transport regulators, and hypothetical proteins, were identified using MS and database search. Twelve of these proteins were apparently reactive to antisera of mice that were immunised with B. pseudomallei secreted proteins. These proteins might be excellent candidates to be used as diagnostic markers or putative candidate vaccines against B. pseudomallei infections.

引用

页码：310 / 320

页数：11

共 59 条

[21] Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei
Holden, MTG
Titball, RW
Peacock, SJ
Cerdeño-Tárraga, AM
Atkins, T
Crossman, LC
Pitt, T
Churcher, C
Mungall, K
Bentley, SD
Sebaihia, M
Thomson, NR
Bason, N
Beacham, IR
Brooks, K
Brown, KA
Brown, NF
Challis, GL
Cherevach, I
Chillingworth, T
Cronin, A
Crossett, B
Davis, P
DeShazer, D
Feltwell, T
Fraser, A
Hance, Z
Hauser, H
Holroyd, S
Jagels, K
Keith, KE
Maddison, M
Moule, S
Price, C
Quail, MA
Rabbinowitsch, E
Rutherford, K
Sanders, M
Simmonds, M
Songsivilai, S
Stevens, K
Tumapa, S
Vesaratchavest, M
Whitehead, S
Yeats, C
Barrell, BG
Oyston, PCF
Parkhill, J
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (39) : 14240 - 14245
[22] Proteomic identification of secreted proteins of Propionibacterium acnes
Holland, Carsten
Mak, Tim N.
Zimny-Arndt, Ursula
Schmid, Monika
Meyer, Thomas F.
Jungblut, Peter R.
Brueggemann, Holger
[J]. BMC MICROBIOLOGY, 2010, 10
[23] Type III protein secretion systems in bacterial pathogens of animals and plants
Hueck, CJ
[J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (02) : 379 - +
[24] Impact of intracellular location of and antigen display by intracellular bacteria: implications for vaccine development
Kaufmann, SHE
Hess, J
[J]. IMMUNOLOGY LETTERS, 1999, 65 (1-2) : 81 - 84
[25] Proteins released by Helicobacter pylori in vitro
Kim, N
Weeks, DL
Shin, JM
Scott, DR
Young, MK
Sachs, G
[J]. JOURNAL OF BACTERIOLOGY, 2002, 184 (22) : 6155 - 6162
[26] Kumar Satish, 1994, Indian Journal of Experimental Biology, V32, P163
[27] Type III secretion systems: Machines to deliver bacterial proteins into eukaryotic cells?
Lee, CA
[J]. TRENDS IN MICROBIOLOGY, 1997, 5 (04) : 148 - 156
[28] In vitro resistance of Burkholderia cepacia complex isolates to reactive oxygen species in relation to catalase and superoxide dismutase production
Lefebre, MD
Valvano, MA
[J]. MICROBIOLOGY-SGM, 2001, 147 : 97 - 109
[29] Lottenberg Richard, 1994, Trends in Microbiology, V2, P20, DOI 10.1016/0966-842X(94)90340-9
[30] Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv
Malen, Hiwa
Berven, Frode S.
Fladmark, Kari E.
Wiker, Harald G.
[J]. PROTEOMICS, 2007, 7 (10) : 1702 - 1718

← 1 2 3 4 5 6 →