Identification of immunoreactive secretory proteins from the stationary phase culture of Burkholderia pseudomallei

被引：15
作者：
Vellasamy, Kumutha Malar [1 ]
Mariappan, Vanitha [1 ]
Hashim, Onn H. [2 ,3 ]
Vadivelu, Jamuna [1 ]
机构：
[1] Univ Malaya, Fac Med, Dept Med Microbiol, Kuala Lumpur 50603, Malaysia
[2] Univ Malaya, Fac Med, Dept Mol Med, Kuala Lumpur 50603, Malaysia
[3] Univ Malaya, Ctr Prote Res, Kuala Lumpur 50603, Malaysia
来源：
ELECTROPHORESIS | 2011年 / 32卷 / 02期
关键词：
Burkholderia pseudomallei; Immunoreactive; Proteomics; Secreted proteins; GROUP-A STREPTOCOCCI; PYRUVATE-DEHYDROGENASE; PROTEOME ANALYSIS; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; MYCOBACTERIUM-TUBERCULOSIS; BACILLUS-SUBTILIS; SURFACE; CELLS; MELIOIDOSIS; ACTIVATION;
D O I：
10.1002/elps.201000355
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Bacterial secreted proteins are known to be involved in virulence and may mediate important host-pathogen interactions. In this study, when the stationary phase culture supernatant of Burkholderia pseudomallei was subjected to 2-DE, 113 protein spots were detected. Fifty-four of the secreted proteins, which included metabolic enzymes, transcription/translation regulators, potential virulence factors, chaperones, transport regulators, and hypothetical proteins, were identified using MS and database search. Twelve of these proteins were apparently reactive to antisera of mice that were immunised with B. pseudomallei secreted proteins. These proteins might be excellent candidates to be used as diagnostic markers or putative candidate vaccines against B. pseudomallei infections.
引用
收藏
页码：310 / 320
页数：11
相关论文
共 59 条
  • [1] PROTEINS RELEASED FROM MYCOBACTERIUM-TUBERCULOSIS DURING GROWTH
    ANDERSEN, P
    ASKGAARD, D
    LJUNGQVIST, L
    BENNEDSEN, J
    HERON, I
    [J]. INFECTION AND IMMUNITY, 1991, 59 (06) : 1905 - 1910
  • [2] The extracellular and cytoplasmic proteomes of the non-virulent Bacillus anthracis strain UM23C1-2
    Antelmann, H
    Williams, RC
    Miethke, M
    Wipat, A
    Albrecht, D
    Harwood, CR
    Hecker, M
    [J]. PROTEOMICS, 2005, 5 (14) : 3684 - 3695
  • [3] Cloning and kinetics of expression of Brucella abortus heat shock proteins by baculovirus recombinants
    Bae, J
    Toth, TE
    [J]. VETERINARY MICROBIOLOGY, 2000, 75 (02) : 199 - 204
  • [4] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [5] ISOLATION AND CHARACTERIZATION OF PSEUDOMONAS-PSEUDOMALLEI FLAGELLIN PROTEINS
    BRETT, PJ
    MAH, DCW
    WOODS, DE
    [J]. INFECTION AND IMMUNITY, 1994, 62 (05) : 1914 - 1919
  • [6] Proteome analysis of secreted proteins of the gastric pathogen Helicobacter pylori
    Bumann, D
    Aksu, S
    Wendland, M
    Janek, K
    Zimny-Arndt, U
    Sabarth, N
    Meyer, TF
    Jungblut, PR
    [J]. INFECTION AND IMMUNITY, 2002, 70 (07) : 3396 - 3403
  • [7] Host cell-dependent secretion and translocation of the LepA and LepB effectors of Legionella pneumophila
    Chen, John
    Reyes, Moraima
    Clarke, Margaret
    Shuman, Howard A.
    [J]. CELLULAR MICROBIOLOGY, 2007, 9 (07) : 1660 - 1671
  • [8] Immunogenicity and anti-Burkholderia pseudomallei activity in Balb/c mice immunized with plasmid DNA encoding flagellin
    Chen, YS
    Hsiao, YS
    Lin, HH
    Yen, CM
    Chen, SC
    Chen, YL
    [J]. VACCINE, 2006, 24 (06) : 750 - 758
  • [9] Distribution of immunoglobulin classes and IgG subclasses against a culture filtrate antigen of Burkholderia pseudomallei in melioidosis patients
    Chenthamarakshan, V
    Kumutha, MV
    Vadivelu, J
    Puthucheary, SD
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2001, 50 (01) : 55 - 61
  • [10] Identification of in vivo-expressed immunogenic proteins by serological proteome analysis of the Bacillus anthracis secretome
    Chitlaru, Theodor
    Gat, Orit
    Grosfeld, Haim
    Inbar, Itzhak
    Gozan, Yael
    Shafferman, Avigdor
    [J]. INFECTION AND IMMUNITY, 2007, 75 (06) : 2841 - 2852
123456