MicroRNA-494 inhibits the growth and angiogenesis-regulating potential of mesenchymal stem cells

被引：47

作者：

Chen, Shiwen ^{[1
]}

Zhao, Guangfeng ^{[1
]}

Miao, Huishuang ^{[2
]}

Tang, Ruijing ^{[2
]}

Song, Yuxian ^{[2
]}

Hu, Yali ^{[1
,3
]}

Wang, Zhiqun ^{[1
]}

Hou, Yayi ^{[2
,3
]}

机构：

[1] Nanjing Univ, Nanjing Drum Tower Hosp, Sch Med, Dept Obstet & Gynecol, Nanjing 210008, Jiangsu, Peoples R China

[2] Nanjing Univ, Sch Med, Div Immunol, State Key Lab Pharmaceut Biotechnol, Nanjing 210008, Jiangsu, Peoples R China

[3] Jiangsu Key Lab Mol Med, Nanjing 210093, Jiangsu, Peoples R China

来源：

FEBS LETTERS | 2015年 / 589卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Preeclampsia; Mesenchymal stem cell; microRNA-494-3p; Vascular endothelial growth factor; Angiogenesis; STROMAL CELLS; DIFFERENTIAL EXPRESSION; SEVERE PREECLAMPSIA; NITRIC-OXIDE; LUNG-CANCER; PART; PROLIFERATION; PREGNANCY; APOPTOSIS; MIR-494;

D O I：

10.1016/j.febslet.2015.01.038

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mesenchymal stem cells (MSCs) play an important role in the pathology of preeclampsia (PE). Our previous microarray analysis found that microRNA-494 (miR-494) is highly expressed in decidua-derived MSCs (dMSCs) from PE. We hypothesized that aberrant expression of miR-494 in dMSCs is involved in PE development. In the present study, we found that miR-494 arrests G1/S transition in dMSCs by targeting CDK6 and CCND1. We also found that supernatant from miR-494-overexpress-ing dMSCs reduces HTR-8/SVneo migration and impairs HUVEC capillary formation by suppressing VEGF. Taken together, we report an unrecognized mechanism of miR-494 affecting dMSC proliferation and function in the pathology of PE. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：710 / 717

页数：8

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