Rapid detection of Salmonella Typhi by loop-mediated isothermal amplification (LAMP) method

被引：29

作者：

Abdullah, J. ^{[1
]}

Saffie, N. ^{[1
]}

Sjasri, F. A. R. ^{[1
]}

Husin, A. ^{[3
]}

Abdul-Rahman, Z. ^{[2
]}

Ismail, A. ^{[1
]}

Aziah, I. ^{[1
]}

Mohamed, M. ^{[1
]}

机构：

[1] Inst Res Mol Med, Kubang Kerian, Kelantan, Malaysia

[2] Univ Sains Malaysia, Sch Med Sci, Kubang Kerian, Kelantan, Malaysia

[3] Hosp Raja Perempuan Zainab II, Kota Baharu 15586, Kelantan, Malaysia

来源：

BRAZILIAN JOURNAL OF MICROBIOLOGY | 2014年 / 45卷 / 04期

关键词：

loop-mediated isothermal amplification (LAMP); Salmonella Typhi; POLYMERASE-CHAIN-REACTION; REVERSE-TRANSCRIPTION; ENTERICA; SAMPLES; FEVER; ASSAY; FOOD; PCR;

D O I：

10.1590/S1517-83822014000400032

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An in-house loop-mediated isothermal amplification (LAMP) reaction was established and evaluated for sensitivity and specificity in detecting the presence of Salmonella Typhi (S. Typhi) isolates from Kelantan, Malaysia. Three sets of primers consisting of two outer and 4 inner were designed based on locus STBHUCCB_38510 of chaperone PapD of S. Typhi genes. The reaction was optimised using genomic DNA of S. Typhi ATCC7251 as the template. The products were visualised directly by colour changes of the reaction. Positive results were indicated by green fluorescence and negative by orange colour. The test was further evaluated for specificity, sensitivity and application on field samples. The results were compared with those obtained by gold standard culture method and Polymerase Chain Reaction (PCR). This method was highly specific and -10 times more sensitive in detecting S. Typhi compared to the optimised conventional polymerase chain reaction (PCR) method.

引用

收藏

页码：1385 / 1391

页数：7

相关论文

共 20 条

[11] A loop-mediated isothermal amplification method targets the phoP gene for the detection of Salmonella in food samples [J].

Li, Xuefei ;

Zhang, Shu ;

Zhang, Hongwei ;

Zhang, Lihuai ;

Tao, Haitao ;

Yu, Jia ;

Zheng, Wenjie ;

Liu, Chenghu ;

Lue, Dan ;

Xiang, Rong ;

Liu, Yin .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2009, 133 (03) :252-258

[12] Enumeration of Salmonella bacteria in food and feed samples by real-time PCR for quantitative microbial risk assessment [J].

Malorny, Burkhard ;

Lofstrom, Charlotta ;

Wagner, Martin ;

Kramer, Nadine ;

Hoorfar, Jeffrey .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (05) :1299-1304

[13] Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation [J].

Mori, Y ;

Nagamine, K ;

Tomita, N ;

Notomi, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 289 (01) :150-154

[14] Loop-mediated isothermal amplification of DNA [J].

Notomi, Tsugunori ;

Okayama, Hiroto ;

Masubuchi, Harumi ;

Yonekawa, Toshihiro ;

Watanabe, Keiko ;

Amino, Nobuyuki ;

Hase, Tetsu .

NUCLEIC ACIDS RESEARCH, 2000, 28 (12)

[15] Detection of Salmonella enterica in naturally contaminated liquid eggs by loop-mediated isothermal amplification, and characterization of Salmonella isolates [J].

Ohtsuka, K ;

Yanagawa, K ;

Takatori, K ;

Hara-Kudo, Y .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (11) :6730-6735

[16] Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay [J].

Parida, M ;

Horioke, K ;

Ishida, H ;

Dash, PK ;

Saxena, P ;

Jana, AM ;

Islam, MA ;

Inoue, S ;

Hosaka, N ;

Morita, K .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (06) :2895-2903

[17] The Rapid Detection of Salmonella from Food Samples By Loop-Mediated Isothermal Amplification (LAMP) [J].

Ueda, Shigeko ;

Kuwabara, Yoshihiro .

BIOCONTROL SCIENCE, 2009, 14 (02) :73-76

[18] Molecular analysis of incHI1 antimicrobial resistance Plasmids from Salmonella serovar typhi strains associated with typhoid fever [J].

Wain, J ;

Nga, LTD ;

Kidgell, C ;

James, K ;

Fortune, S ;

Diep, TS ;

Ali, T ;

Gaora, PO ;

Parry, C ;

Parkhill, J ;

Farrar, J ;

White, NJ ;

Dougan, G .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (09) :2732-2739

[19] Evaluation of the Premi®Test Salmonella, a commercial low-density DNA microarray system intended for routine identification and typing of Salmonella enterica [J].

Wattiau, Pierre ;

Weijers, Thijs ;

Andreoli, Peter ;

Schliker, Christine ;

Veken, Heidi Vander ;

Maas, Henny M. E. ;

Verbruggen, Anjo J. ;

Heck, Max E. O. C. ;

Wannet, Wim J. ;

Imberechts, Hein ;

Vos, Pieter .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2008, 123 (03) :293-298

[20] Detection of Salmonella typhi by polymerase chain reaction [J].

Zhu, Q ;

Lim, CK ;

Chan, YN .

JOURNAL OF APPLIED BACTERIOLOGY, 1996, 80 (03) :244-251