An in-house loop-mediated isothermal amplification (LAMP) reaction was established and evaluated for sensitivity and specificity in detecting the presence of Salmonella Typhi (S. Typhi) isolates from Kelantan, Malaysia. Three sets of primers consisting of two outer and 4 inner were designed based on locus STBHUCCB_38510 of chaperone PapD of S. Typhi genes. The reaction was optimised using genomic DNA of S. Typhi ATCC7251 as the template. The products were visualised directly by colour changes of the reaction. Positive results were indicated by green fluorescence and negative by orange colour. The test was further evaluated for specificity, sensitivity and application on field samples. The results were compared with those obtained by gold standard culture method and Polymerase Chain Reaction (PCR). This method was highly specific and -10 times more sensitive in detecting S. Typhi compared to the optimised conventional polymerase chain reaction (PCR) method.
机构:
Univ Sains Malaysia, Inst Res Mol Med, Kubang Kerian 16150, Kelantan, MalaysiaUniv Sains Malaysia, Inst Res Mol Med, Kubang Kerian 16150, Kelantan, Malaysia
Aziah, Ismail
Ravichandran, Manickam
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Univ Sains Malaysia, Sch Med Sci, Dept Med Microbiol & Parasitol, Kubang Kerian 16150, Kelantan, MalaysiaUniv Sains Malaysia, Inst Res Mol Med, Kubang Kerian 16150, Kelantan, Malaysia
Ravichandran, Manickam
Ismail, Asma
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机构:
Univ Sains Malaysia, Inst Res Mol Med, Kubang Kerian 16150, Kelantan, MalaysiaUniv Sains Malaysia, Inst Res Mol Med, Kubang Kerian 16150, Kelantan, Malaysia
[3]
Communicable Disease Control Section Communicable Disease Control Section Disease Control Division Ministry of Health Malaysia, 2008, TYPH TYPH FEV
机构:
Ctr Dis Control & Prevent, Branch Lab, Div HIV AIDS Prevent, Natl Ctr HIV AIDS Hepatitis STD & TB Prevent, Atlanta, GA 30333 USACtr Dis Control & Prevent, Branch Lab, Div HIV AIDS Prevent, Natl Ctr HIV AIDS Hepatitis STD & TB Prevent, Atlanta, GA 30333 USA
Curtis, Kelly A.
Rudolph, Donna L.
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Ctr Dis Control & Prevent, Branch Lab, Div HIV AIDS Prevent, Natl Ctr HIV AIDS Hepatitis STD & TB Prevent, Atlanta, GA 30333 USACtr Dis Control & Prevent, Branch Lab, Div HIV AIDS Prevent, Natl Ctr HIV AIDS Hepatitis STD & TB Prevent, Atlanta, GA 30333 USA
Rudolph, Donna L.
Owen, S. Michele
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h-index: 0
机构:
Ctr Dis Control & Prevent, Branch Lab, Div HIV AIDS Prevent, Natl Ctr HIV AIDS Hepatitis STD & TB Prevent, Atlanta, GA 30333 USACtr Dis Control & Prevent, Branch Lab, Div HIV AIDS Prevent, Natl Ctr HIV AIDS Hepatitis STD & TB Prevent, Atlanta, GA 30333 USA
机构:
Kanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, Japan
Eiken Chem Co, Ohtawara, Tochigi, JapanKanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, Japan
Hosaka, Norimitsu
Ndembi, Nicaise
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Kanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, Japan
Univ Yaounde I, Fac Med & Biomed Sci, Lab Hematol & Virol, Yaounde, CameroonKanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, Japan
Ndembi, Nicaise
Ishizaki, Azumi
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Kanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, JapanKanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, Japan
Ishizaki, Azumi
Kageyama, Seiji
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Kanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, JapanKanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, Japan
Kageyama, Seiji
Numazaki, Kei
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机构:Kanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, Japan
Numazaki, Kei
Ichimura, Hiroshi
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Kanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, JapanKanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, Japan
机构:
Univ Sains Malaysia, Inst Res Mol Med, Kubang Kerian 16150, Kelantan, MalaysiaUniv Sains Malaysia, Inst Res Mol Med, Kubang Kerian 16150, Kelantan, Malaysia
Aziah, Ismail
Ravichandran, Manickam
论文数: 0引用数: 0
h-index: 0
机构:
Univ Sains Malaysia, Sch Med Sci, Dept Med Microbiol & Parasitol, Kubang Kerian 16150, Kelantan, MalaysiaUniv Sains Malaysia, Inst Res Mol Med, Kubang Kerian 16150, Kelantan, Malaysia
Ravichandran, Manickam
Ismail, Asma
论文数: 0引用数: 0
h-index: 0
机构:
Univ Sains Malaysia, Inst Res Mol Med, Kubang Kerian 16150, Kelantan, MalaysiaUniv Sains Malaysia, Inst Res Mol Med, Kubang Kerian 16150, Kelantan, Malaysia
[3]
Communicable Disease Control Section Communicable Disease Control Section Disease Control Division Ministry of Health Malaysia, 2008, TYPH TYPH FEV
机构:
Ctr Dis Control & Prevent, Branch Lab, Div HIV AIDS Prevent, Natl Ctr HIV AIDS Hepatitis STD & TB Prevent, Atlanta, GA 30333 USACtr Dis Control & Prevent, Branch Lab, Div HIV AIDS Prevent, Natl Ctr HIV AIDS Hepatitis STD & TB Prevent, Atlanta, GA 30333 USA
Curtis, Kelly A.
Rudolph, Donna L.
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Dis Control & Prevent, Branch Lab, Div HIV AIDS Prevent, Natl Ctr HIV AIDS Hepatitis STD & TB Prevent, Atlanta, GA 30333 USACtr Dis Control & Prevent, Branch Lab, Div HIV AIDS Prevent, Natl Ctr HIV AIDS Hepatitis STD & TB Prevent, Atlanta, GA 30333 USA
Rudolph, Donna L.
Owen, S. Michele
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Dis Control & Prevent, Branch Lab, Div HIV AIDS Prevent, Natl Ctr HIV AIDS Hepatitis STD & TB Prevent, Atlanta, GA 30333 USACtr Dis Control & Prevent, Branch Lab, Div HIV AIDS Prevent, Natl Ctr HIV AIDS Hepatitis STD & TB Prevent, Atlanta, GA 30333 USA
机构:
Kanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, Japan
Eiken Chem Co, Ohtawara, Tochigi, JapanKanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, Japan
Hosaka, Norimitsu
Ndembi, Nicaise
论文数: 0引用数: 0
h-index: 0
机构:
Kanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, Japan
Univ Yaounde I, Fac Med & Biomed Sci, Lab Hematol & Virol, Yaounde, CameroonKanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, Japan
Ndembi, Nicaise
Ishizaki, Azumi
论文数: 0引用数: 0
h-index: 0
机构:
Kanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, JapanKanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, Japan
Ishizaki, Azumi
Kageyama, Seiji
论文数: 0引用数: 0
h-index: 0
机构:
Kanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, JapanKanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, Japan
Kageyama, Seiji
Numazaki, Kei
论文数: 0引用数: 0
h-index: 0
机构:Kanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, Japan
Numazaki, Kei
Ichimura, Hiroshi
论文数: 0引用数: 0
h-index: 0
机构:
Kanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, JapanKanazawa Univ, Grad Sch Med Sci, Dept Viral Infect & Int Hlth, Kanazawa, Ishikawa 9208640, Japan