Rapid detection of Salmonella Typhi by loop-mediated isothermal amplification (LAMP) method

被引：27
作者：
Abdullah, J. [1 ]
Saffie, N. [1 ]
Sjasri, F. A. R. [1 ]
Husin, A. [3 ]
Abdul-Rahman, Z. [2 ]
Ismail, A. [1 ]
Aziah, I. [1 ]
Mohamed, M. [1 ]
机构：
[1] Inst Res Mol Med, Kubang Kerian, Kelantan, Malaysia
[2] Univ Sains Malaysia, Sch Med Sci, Kubang Kerian, Kelantan, Malaysia
[3] Hosp Raja Perempuan Zainab II, Kota Baharu 15586, Kelantan, Malaysia
来源：
BRAZILIAN JOURNAL OF MICROBIOLOGY | 2014年 / 45卷 / 04期
关键词：
loop-mediated isothermal amplification (LAMP); Salmonella Typhi; POLYMERASE-CHAIN-REACTION; REVERSE-TRANSCRIPTION; ENTERICA; SAMPLES; FEVER; ASSAY; FOOD; PCR;
D O I：
10.1590/S1517-83822014000400032
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
An in-house loop-mediated isothermal amplification (LAMP) reaction was established and evaluated for sensitivity and specificity in detecting the presence of Salmonella Typhi (S. Typhi) isolates from Kelantan, Malaysia. Three sets of primers consisting of two outer and 4 inner were designed based on locus STBHUCCB_38510 of chaperone PapD of S. Typhi genes. The reaction was optimised using genomic DNA of S. Typhi ATCC7251 as the template. The products were visualised directly by colour changes of the reaction. Positive results were indicated by green fluorescence and negative by orange colour. The test was further evaluated for specificity, sensitivity and application on field samples. The results were compared with those obtained by gold standard culture method and Polymerase Chain Reaction (PCR). This method was highly specific and -10 times more sensitive in detecting S. Typhi compared to the optimised conventional polymerase chain reaction (PCR) method.
引用
收藏
页码：1385 / 1391
页数：7
相关论文
共 20 条
  • [1] [Anonymous], 2003, 65792003 ISO
  • [2] Amplification of ST50 gene using dry-reagent-based polymerase chain reaction for the detection of Salmonella typhi
    Aziah, Ismail
    Ravichandran, Manickam
    Ismail, Asma
    [J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2007, 59 (04) : 373 - 377
  • [3] Communicable Disease Control Section Communicable Disease Control Section Disease Control Division Ministry of Health Malaysia, 2008, TYPH TYPH FEV
  • [4] Rapid detection of HIV-1 by reverse-transcription, loop-mediated isothermal amplification (RT-LAMP)
    Curtis, Kelly A.
    Rudolph, Donna L.
    Owen, S. Michele
    [J]. JOURNAL OF VIROLOGICAL METHODS, 2008, 151 (02) : 264 - 270
  • [5] Use of loop-mediated isothermal amplification of the IS900 sequence for rapid detection of cultured Mycobacterium avium subsp. paratuberculosis
    Enosawa, M
    Kageyama, S
    Sawai, K
    Watanabe, K
    Notomi, T
    Onoe, S
    Mori, Y
    Yokomizo, Y
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (09) : 4359 - 4365
  • [6] Loop-mediated isothermal amplification for the rapid detection of Salmonella
    Hara-Kudo, Y
    Yoshino, M
    Kojima, T
    Ikedo, M
    [J]. FEMS MICROBIOLOGY LETTERS, 2005, 253 (01) : 155 - 161
  • [7] Sensitive and specific detection of Yersinia pseudotuberculosis by loop-mediated isothermal amplification
    Horisaka, T
    Fujita, K
    Iwata, T
    Nakadai, A
    Okatani, AT
    Horikita, T
    Taniguchi, T
    Honda, E
    Yokomizo, Y
    Hayashidani, H
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (11) : 5349 - 5352
  • [8] Rapid detection of human immunodeficiency virus type 1 group M by a reverse transcription-loop-mediated isothermal amplification assay
    Hosaka, Norimitsu
    Ndembi, Nicaise
    Ishizaki, Azumi
    Kageyama, Seiji
    Numazaki, Kei
    Ichimura, Hiroshi
    [J]. JOURNAL OF VIROLOGICAL METHODS, 2009, 157 (02) : 195 - 199
  • [9] Detection of fish nocardiosis by loop-mediated isothermal amplification
    Itano, T.
    Kawakami, H.
    Kono, T.
    Sakai, M.
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2006, 100 (06) : 1381 - 1387
  • [10] PCR method to identify Salmonella enterica serovars Typhi, paratyphi A, and paratyphi B among salmonella isolates from the blood of patients with clinical enteric fever
    Levy, Haim
    Diallo, Souleymane
    Tennant, Sharon M.
    Livio, Sofie
    Sow, Samba O.
    Tapia, Milagritos
    Fields, Patricia I.
    Mikoleit, Matthew
    Tamboura, Boubou
    Kotloff, Karen L.
    Lagos, Rosanna
    Nataro, James P.
    Galen, James E.
    Levine, Myron M.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (05) : 1861 - 1866
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