The 2000-2001 GEP-ISFG Collaborative Exercise on mtDNA:: assessing the cause of unsuccessful mtDNA PCR amplification of hair shaft samples

被引：27

作者：

Prieto, L ^{[1
]}

Montesino, M

Salas, A

Alonso, A

Albarrán, C

Alvarez, S

Crespillo, M

Di Lonardo, AM

Doutremepuich, C

Fernández-Fernández, I

de la Vega, AG

Gusmao, L

López, CM

López-Soto, M

Lorente, JA

Malaghini, M

Martínez, CA

Modesti, NM

Palacio, AM

Paredes, M

Pena, SDJ

Pérez-Lezaun, A

Pestano, JJ

Puente, J

Sala, A

Vide, MC

Whittle, MR

Yunis, JJ

Gómez, J

机构：

[1] Comisaria Gen Policia Cient, Secc Biol ADN, Madrid, Spain

[2] Univ Santiago Compostela, Inst Med Legale, Fac Med, Coruna, Spain

[3] Inst Toxicol, Dept Madrid, Secc Biol, Madrid, Spain

[4] Univ Complutense Madrid, Fac Med, Madrid, Spain

[5] Inst Toxicol, Dept Barcelona, Secc Biol, Barcelona, Spain

[6] Hosp Dr C G Durand, Banco Nacl Datos Gen, Buenos Aires, DF, Argentina

[7] Hematol Lab, Bordeaux, France

[8] DataGene, Bizkaia, Spain

[9] ADF TecnoGen SL, Madrid, Spain

[10] Univ Porto, Inst Patol & Immunol, Oporto, Portugal

[11] Direcc Gen Guardia Civil, Lab ADN, Madrid, Spain

[12] Inst Toxicol, Dept Sevilla, Secc Biol, Seville, Spain

[13] Univ Granada, Dept Legal Med, Lab Identificac Genet, Granada, Spain

[14] Inst Criminalist, Lab Genet Mol Forense Curitiva, Parana, Brazil

[15] Lab Anal Clin Frischmann, Curitiba, Parana, Brazil

[16] CEPROCOR, Cordoba, Argentina

[17] Cent Anal Genet, Zaragoza, Spain

[18] Inst Nacl Med Legal & Ciencias Forenses, Lab DNA, Bogota, Colombia

[19] GENE, Nucleo Genet Medica, Belo Horizonte, MG, Brazil

[20] Univ Pompeu Fabra, Unitat Biol Evolutiva, Barcelona, Spain

[21] Inst Anat Forense, Fac Med, Genet Lab, Las Palmas Gran Canaria, Spain

[22] Dept Biol Mol Pharma Gen SA, Madrid, Spain

[23] Univ Buenos Aires, Fac Farm & Bioquim, Serv Huellas Digitales Genet, Buenos Aires, DF, Argentina

[24] Inst Med Legale, Serv Biol Forense, Coimbra, Portugal

[25] Genom Engn Mol LTDA, Sao Paulo, Brazil

[26] Serv Med Yunis Turbay & Cia, Bogota, DC, Colombia

[27] Inst Toxicol, Unidad Garantia Calidad, Madrid, Spain

来源：

FORENSIC SCIENCE INTERNATIONAL | 2003年 / 134卷 / 01期

关键词：

mitochondrial DNA (mtDNA) polymorphism; standardization; collaborative exercise; heteroplasmy; hair shafts; annual identification; cytochrome b; 12S; 16S;

D O I：

10.1016/S0379-0738(03)00095-1

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

We report the results of Spanish and Portuguese working group (GEP) of International Society of Forensic Genetics (ISFG) Collaborative Exercise 2001-2002 on mitochondrial DNA (mtDNA) analysis. 64 laboratories from Spain, Portugal and several Latin-American countries participated in this quality control exercise. Five samples were sent to the participating laboratories, four blood stains (M1-M4) and a sample (M5) consisting of two hair shaft fragments. M4 was non-human (Felis catus) in origin; therefore. the capacity of the labs to identify the biological source of this sample was an integral part of the exercise. Some labs detected the non-human origin of M4 by carrying out immuno-diffussion techniques using antihuman serum, whereas others identified the specific animal origin by testing the sample against a set of animal antibodies or by means of the analysis of mtDNA regions (Cyt-b, 12S, and 16S genes). The results of the other three human blood stains (M1-M3) improved in relation to the last Collaborative Exercises but those related to hairs yielded a low rate of success which clearly contrasts with previous results. As a consequence of this, some labs performed additional analysis showing that the origin of this low efficiency was not the presence of inhibitors, but the low quantity of DNA present in these specific hair samples and the degradation. As a general conclusion the results emphasize the need of external proficiency testing as part of the accreditation procedure for the labs performing mtDNA analysis in forensic casework. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：46 / 53

页数：8

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