Partitioning of Canavalia brasiliensis Lectin in Polyethylene Glycol - Sodium Citrate Aqueous Two-Phase Systems

被引：11
作者：
Nascimento, Kelany S. [1 ]
Azevedo, A. M. [1 ]
Cavada, B. S. [2 ]
Aires-Barros, M. R. [1 ]
机构：
[1] Inst Super Tecn, Ctr Biol & Chem Engn, IBB, P-1000 Lisbon, Portugal
[2] Univ Fed Ceara, Biochem & Mol Biol Dept, Fortaleza, Ceara, Brazil
来源：
SEPARATION SCIENCE AND TECHNOLOGY | 2010年 / 45卷 / 15期
关键词：
aqueous two-phase systems; Canavalia brasiliensis; lectin; polyethylene glycol; sodium citrate; PURIFICATION; EXTRACTION; OPTIMIZATION; FEATURES;
D O I：
10.1080/01496395.2010.507446
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The partitioning of Canavalia brasiliensis (ConBr) lectin from Canavalia brasiliensis seeds was carried out in aqueous two-phase systems composed of polyethylene glycol (PEG) and sodium citrate. The effect of PEG molecular weight (600, 1000, 2000, and 3350), pH (6.0, 7.0, and 8.0) and ionic strength (0, 2, 5, and 10% NaCl) on the partition behavior of ConBr in ATPS was systematically investigated. Liquid-liquid equilibrium data for the PEG 600-sodium citrate system at pH 6.0, 7.0, and 8.0 were determined at 25 degrees C. The effect of the pH on the position of the binodal curves was not significant, but it had a strong influence in the partitioning of the ConBr. An increase in the NaCl concentration significantly favored the partitioning of ConBr to the top phase, probably due the dehydration of the protein molecules triggered by high ionic strength. The best system for purification of ConBr was composed by 17.54% PEG 600, 15.14% sodium citrate, 5% NaCl at pH 8.0 with recovery yield 70% and 98% purity. This system was found suitable for the recovery of ConBr, since it not only allowed the partitioning of the lectin towards the PEG-rich phase but also the concentration of contaminants in the salt-rich phase.
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收藏
页码：2180 / 2186
页数：7
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