Optimized cleanup methods of organic extracts for the determination of organic pollutants in biological samples

被引：27

作者：

Fuoco, R ^{[1
]}

Giannarelli, S ^{[1
]}

Onor, M ^{[1
]}

Ceccarini, A ^{[1
]}

Carli, V ^{[1
]}

机构：

[1] Univ Pisa, Dept Chem & Ind Chem, I-56126 Pisa, Italy

来源：

MICROCHEMICAL JOURNAL | 2005年 / 79卷 / 1-2期

关键词：

cleanup; organic extract; silica gel-alumina;

D O I：

10.1016/j.microc.2004.06.009

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The analytical performances of various adsorbents used to clean up an organic extract of biological samples, particularly for the determination of polycyclic aromatic hydrocarbons (PAHs), polychlorobiphenyls (PCBs), both in mussel tissue and in krill samples, are critically compared. DDT and its degradation products, namely DDE and DDD, are also considered. Silica gel, alumina, armnopropyl-silica, cyanopropyl-silica, florisil, graphitized nonporous carbon and silica gel-alumina mixture (3:1) were used for column chromatography cleanup in combination with modified supercritical CO2 as a mobile phase. Recovery and reproducibility were evaluated by analyzing standard solutions and standard reference materials containing various classes of pollutants. A silica gel-alumina mixture was found to be the most effective in the cleanup of organic extracts, allowing quantitative recoveries of all analytes to be obtained. Finally, the application of the optimized procedure to the determination of PCBs in Antarctic krill samples is presented. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：69 / 76

页数：8

共 48 条

[1] Evaluation of a new automated cleanup system for the analysis of polychlorinated dibenzo-p-dioxins and dibenzofurans in environmental samples [J].