Fluorescence switch for silver ion detection utilizing dimerization of DNA-Ag nanoclusters

被引：76

作者：

Lee, Jihyun ^{[1
,2
]}

Park, Juhee ^{[1
,2
]}

Lee, Hong Hee ^{[3
]}

Park, Hansoo ^{[4
]}

Kim, Hugh I. ^{[3
]}

Kim, Won Jong ^{[1
,2
]}

机构：

[1] Pohang Univ Sci & Technol POSTECH, Inst Basic Sci, Ctr Self Assembly & Complex, Pohang 790784, South Korea

[2] Pohang Univ Sci & Technol POSTECH, Dept Chem, Pohang 790784, South Korea

[3] Pohang Univ Sci & Technol POSTECH, Dept Chem, Div Adv Mat Sci, Pohang 790784, South Korea

[4] Chung Ang Univ, Sch Integrat Engn, Seoul 156756, South Korea

来源：

BIOSENSORS & BIOELECTRONICS | 2015年 / 68卷

基金：

新加坡国家研究基金会;

关键词：

DNA-template silver nanoclusters; Silver ion detection; Fluorescent probes; Fluorescence switch; BASE-PAIRS; NANOPARTICLES; ARGYRIA; PROBE;

D O I：

10.1016/j.bios.2015.01.058

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A fluorescence switch that consists of DNA-templated silver nanoclusters (DNA-AgNCs) triggered by silver ion (Ag+) is developed to detect Ag+. The mechanism of the fluorescence switching of DNA-AgNCs is investigated by fluorescence spectroscopy, circular dichroism spectroscopy, DNA hybridization assay and mass spectrometry. Ag+ induces a dimeric structure of Cyt(12)-AgNCs by forming a bridge between two Cyt(12)-AgNCs, where Cyt(12) is cytosine 12-mer; this dimer formation causes the fluorescence change of Cyt(12)-AgNCs from red to green. Using this Ag+-triggered fluorescence switch, we successfully detected Ag+ at concentrations as low as 10 nM. Furthermore, we quantitatively detected the Ag+ in the Silmazin (R), which is dermatological burn ointment having silver sulfadiazine. Ag+ detection using this fluorescence switch has high selectivity and sensitivity, and short response time, and can be used successfully even in the presence of other metal ions. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：642 / 647

页数：6

共 29 条

[1] Metal ion chelation in neurodegenerative disorders [J].