Synthesis of new fluorogenic cyanine dyes and incorporation into RNA fluoromodules

被引：123

作者：

Constantin, Tudor P. ^{[1
]}

Silva, Gloria L. ^{[1
]}

Robertson, Kelly L. ^{[1
]}

Hamilton, Tamara P. ^{[1
]}

Fague, Kaitlin ^{[1
]}

Waggoner, Alan S. ^{[2
]}

Armitage, Bruce A. ^{[1
,2
]}

机构：

[1] Carnegie Mellon Univ, Dept Chem, Pittsburgh, PA 15213 USA

[2] Carnegie Mellon Univ, Mol Biosensor & Imaging Ctr, Pittsburgh, PA 15213 USA

来源：

ORGANIC LETTERS | 2008年 / 10卷 / 08期

关键词：

D O I：

10.1021/ol702920e

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A new fluorogenic cyanine dye was synthesized and found to have low fluorescence quantum yield in fluid solution and in the presence of double-stranded DNA but 80-fold enhanced fluorescence in viscous glycerol solution. An RNA aptamer selected for binding to the new dye exhibits K-d = 87 nM and 60-fold fluorescence enhancement. The dye-aptamer pair is a fluoromodule that can be incorporated into fluorescent sensors and labels.

引用

页码：1561 / 1564

页数：4

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