Detection of antibodies against Pasteurella multocida using immunohistochemical staining in an outbreak of rabbit pasteurellosis

To detect serum antibody against Pasteurella multocida (P.multocida) in infected rabbits, a modified immunoperoxidase assay was applied. An outbreak of P.multocida infection in rabbits started from sudden death. The infected rabbits had severe fibrinous and purulent pneumonia with hemorrhage, and a large number of P.multocida (A:12) was isolated from the trachea and lungs of the animals. Antibodies of IgM and IgG to P.multocida were assessed by immunohistochemical staining using the sera of the animals as primary antibodies and applying them to formalin-fixed, paraffin-embedded sections of P.multocida attached to calf fibrin. IgM antibodies to P.multocida were first detected 7 days after the onset of the disease. IgG antibodies began to rise on the 7th or 14th day. These results suggested that the modified immunoperoxidase assay could detect antibodies against P.multocida.