Time-resolved all fiber fluorescence spectroscopy system

被引：0

作者：

Chen, A. Y. H. ^{[1
]}

Vanholsbeeck, F. ^{[1
]}

Tai, D. C. S.

Svrcek, M. ^{[2
,3
]}

Smaill, B. H. ^{[2
]}

机构：

[1] Univ Auckland, Dept Phys, Auckland 1, New Zealand

[2] Univ Auckland, Dept Physiol, Auckland 1, New Zealand

[3] Brno Univ Technol, Dept Biomed Engn, Brno, Czech Republic

来源：

OPTICAL FIBERS AND SENSORS FOR MEDICAL DIAGNOSTICS AND TREATMENT APPLICATIONS X | 2010年 / 7559卷

关键词：

action potential; optical fiber probe; motion artifact; di-4-ANEPPS; membrane potential; optical mapping;

D O I：

10.1117/12.840811

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We describe a simple fiber optic fluorescence spectrometry system with a wide variety of biomedical applications. This low-cost, all-fiber system is portable, robust and has the capacity to acquire fluorescence spectra at rates up to 1 kHz. We demonstrate the capabilities of the system by presenting experimental measurements of action potentials in the di-4-ANEPPS stained rat heart using spectral analysis.

引用

页数：6

共 7 条

[1] SUPPRESSION OF MOTION ARTIFACTS IN FLUORESCENCE SPECTROSCOPY OF PERFUSED HEARTS [J].

BRANDES, R ;

FIGUEREDO, VM ;

CAMACHO, SA ;

MASSIE, BM ;

WEINER, MW .

AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 263 (03) :H972-H980

[2] High-speed, random-access fluorescence microscopy: II. Fast quantitative measurements with voltage-sensitive dyes [J].

Bullen, A ;

Saggau, P .

BIOPHYSICAL JOURNAL, 1999, 76 (04) :2272-2287

[3] Ratiometric intracellular calcium imaging in the isolated beating rat heart using indo-1 fluorescence [J].

Eerbeek, O ;

Mik, EG ;

Zuurbier, CJ ;

van 't Loo, M ;

Donkersloot, C ;

Ince, C .

JOURNAL OF APPLIED PHYSIOLOGY, 2004, 97 (06) :2042-2050

[4] Intramural multisite recording of transmembrane potential in the heart [J].

Hooks, DA ;

LeGrice, IJ ;

Harvey, JD ;

Smaill, BH .

BIOPHYSICAL JOURNAL, 2001, 81 (05) :2671-2680

[5] Ratiometry of transmembrane voltage-sensitive fluorescent dye emission in hearts [J].

Knisley, SB ;

Justice, RK ;

Kong, W ;

Johnson, PL .

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2000, 279 (03) :H1421-H1433

[6] DESIGN AND USE OF AN OPTRODE FOR OPTICAL RECORDINGS OF CARDIAC ACTION-POTENTIALS [J].

NEUNLIST, M ;

ZOU, SZ ;

TUNG, L .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1992, 420 (5-6) :611-617

[7] Illumination and fluorescence collection volumes for fiber optic probes in tissue [J].

Tai, Dean C. S. ;

Hooks, Darren A. ;

Harvey, John D. ;

Smaill, Bruce H. ;

Soeller, Christian .

JOURNAL OF BIOMEDICAL OPTICS, 2007, 12 (03)

← 1 →