Impact of carbon and nitrogen feeding strategy on high production of biomass and docosahexaenoic acid (DHA) by Schizochytrium sp LU310

被引：86

作者：

Ling, Xueping ^{[1
]}

Guo, Jing ^{[1
]}

Liu, Xiaoting ^{[1
]}

Zhang, Xia ^{[1
]}

Wang, Nan ^{[1
]}

Lu, Yinghua ^{[1
]}

Ng, I-Son ^{[1
,2
]}

机构：

[1] Xiamen Univ, Coll Chem & Chem Engn, Dept Chem & Biochem Engn, Xiamen 361005, Peoples R China

[2] Natl Cheng Kung Univ, Dept Chem Engn, Tainan 70101, Taiwan

来源：

BIORESOURCE TECHNOLOGY | 2015年 / 184卷

关键词：

Docosahexaenoic acid (DHA); Schizochytrium sp; Baffled flasks; Nitrogen feeding; Carbon feeding; OXYGEN-TRANSFER COEFFICIENT; CELL-DENSITY CULTIVATION; LIMACINUM SR21; FATTY-ACID; SP S31; LIPID-ACCUMULATION; FERMENTATION; CULTURE; MICROORGANISMS; GLUTAMATE;

D O I：

10.1016/j.biortech.2014.09.130

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

A new isolated Schizochytrium sp. LU310 from the mangrove forest of Wenzhou, China, was found as a high producing microalga of docosahexaenoic acid (DHA). In this study, the significant improvements for DHA fermentation by the batch mode in the baffled flasks (i.e. higher oxygen supply) were achieved. By applied the nitrogen-feeding strategy in 1000 mL baffled flasks, the biomass, DHA concentration and DHA productivity were increased by 110.4%, 117.9% and 110.4%, respectively. Moreover, DHA concentration of 21.06 g/L was obtained by feeding 15 g/L of glucose intermittently, which was an increase of 41.25% over that of the batch mode. Finally, an innovative strategy was carried out by intermittent feeding carbon and simultaneously feeding nitrogen. The maximum DHA concentration and DHA productivity in the fed-batch cultivation reached to 24.74 g/L and 241.5 mg/L/h, respectively. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：139 / 147

页数：9

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