Raman Imaging Spectroscopy as a Tool To Investigate the Cell Damage on Aspergillus ochraceus Caused by an Antimicrobial Packaging Containing Benzyl Isothiocyanate

被引:21
作者
Clemente, Isabel [1 ]
Aznar, Margarita [1 ]
Nerin, Cristina [1 ]
机构
[1] Univ Zaragoza, Grp GUIA, Dept Quim Analit, Inst Invest Ingn Aragon I3A, E-50018 Zaragoza, Spain
关键词
ALLYL ISOTHIOCYANATE; RELEVANT MICROORGANISMS; ESCHERICHIA-COLI; ESSENTIAL OIL; IDENTIFICATION; OCHRATOXIN; CINNAMON; EXPOSURE; FOODS; MICROSPECTROSCOPY;
D O I
10.1021/acs.analchem.6b00116
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Raman imaging spectroscopy is a nondestructive analytical method that can be a useful tool to obtain detailed information about the molecular composition and morphology of biological samples. Its high spatial resolution was used to collect spectra of Aspergillus ochraceus, a mold producer of ochratoxin A (OTA), in order to investigate the cell damage caused on it by the action of the antimicrobial benzyl isothiocyanate (BITC). The study was performed in both direct contact and vapor phase, in order to check the use of BITC as active agent in food packaging material. The results showed that there were morphologic alteration and a characteristic Raman spectrum on spore and hyphae exposed to BITC. BITC was accumulated in the mold cells where it caused an enormous amount of alterations in cellular components (lipids, proteins, saccharides, amino acids...) and cellular functions (cell cycle, respiration, metabolism, transcription of genes, fluidity of the cellular wall). All these structural, composition, and metabolic changes will affect the production of OTA. Pattern recognition with chemometrics using principal component analysis (PCA) demonstrated an excellent separation between control and BITC treated samples, both in spores and hyphae. PCA results also showed two different affection levels when samples were exposed to BITC in the vapor phase.
引用
收藏
页码:4772 / 4779
页数:8
相关论文
共 65 条
[1]   OPINION OF THE SCIENTIFIC PANEL ON CONTAMINANTS IN THE FOOD CHAIN ON A REQUEST FROM THE COMMISSION RELATED TO OCHRATOXIN A IN FOOD [J].
Alexander, Jan ;
Autrup, Herman ;
Bard, Denis ;
Benford, Diane ;
Carere, Angelo ;
Costa, Lucio Guido ;
Cravedi, Jean-Pierre ;
Di Domenico, Alessandro ;
Fanelli, Roberto ;
Fink-Gremmels, Johanna ;
Gilbert, John ;
Grandjean, Philippe ;
Johansson, Niklas ;
Oskarsson, Agneta ;
Ruprich, Jiri ;
Schlatter, Josef ;
Schoeters, Greet ;
Schrenk, Dieter ;
van Leeuwen, Rolaf ;
Verger, Philippe .
EFSA JOURNAL, 2006, 4 (06)
[2]  
[Anonymous], 2008, EFSA J, V793, P1
[3]  
[Anonymous], 2004, EFSAJOURNAL, V101, P1, DOI [DOI 10.1029/RG001I004P00507, 10.2903/j.efsa.2004.101, DOI 10.2903/J.EFSA.2004.101]
[4]   Surface-enhanced Raman spectroscopy of DNA [J].
Barhoumi, Aoune ;
Zhang, Dongmao ;
Tam, Felicia ;
Halas, Naomi J. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (16) :5523-5529
[5]   Combination of analytical and microbiological techniques to study the antimicrobial activity of a new active food packaging containing cinnamon or oregano against E-coli and S-aureus [J].
Becerril, R. ;
Gomez-Lus, R. ;
Goni, P. ;
Lopez, P. ;
Nerin, C. .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 388 (5-6) :1003-1011
[6]   Reactions of allyl isothiocyanate with alanine, glycine, and several peptides in model systems [J].
Cejpek, K ;
Valusek, J ;
Velusek, J .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (08) :3560-3565
[7]   Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells [J].
Chan, JW ;
Taylor, DS ;
Zwerdling, T ;
Lane, SM ;
Ihara, K ;
Huser, T .
BIOPHYSICAL JOURNAL, 2006, 90 (02) :648-656
[8]   Micro-Raman spectroscopy used to identify and grade human skin pilomatrixoma [J].
Cheng, WT ;
Liu, MT ;
Liu, HN ;
Lin, SY .
MICROSCOPY RESEARCH AND TECHNIQUE, 2005, 68 (02) :75-79
[9]   The influence of intracellular storage material on bacterial identification by means of Raman spectroscopy [J].
Ciobota, Valerian ;
Burkhardt, Eva-Maria ;
Schumacher, Wilm ;
Roesch, Petra ;
Kuesel, Kirsten ;
Popp, Juergen .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 397 (07) :2929-2937
[10]   Raman imaging of floating cells [J].
Creely, CM ;
Volpe, G ;
Singh, GP ;
Soler, M ;
Petrov, DV .
OPTICS EXPRESS, 2005, 13 (16) :6105-6110