In this study, sequential selective extraction was used to study the influence of sludge concentration of 2% similar to 10%, while using S and FeSO(4)center dot 7H(2)O as the substrate, on bioleaching of heavy metals from sewage sludge. Experimental results show that Cu and Zn removal rate was different while using different sludge concentration after leaching 14 days, and the lower of sludge concentration, the Cu, Zn removal rate was higher. Using S as the substrate, the removal rate of Cu was highest while 6% solids concentration in the sludge samples, and the removal rate was 10.1%. 4% solids concentration in the sludge samples Zn removal of 24.9%. Using FeSO(4)center dot 7H(2)O as the substrate, the 2% solid concentration had the highest Cu, Zn removal rate, and the removal rate was 31.4% and 34.7%, respectively. Cu and Zn hand the best bioleaching results when the solid concentration was 4% in this research.