Dye-sensitized biosystem sensing using macroporous semiconducting metal oxide films

被引：38

作者：

Chandra, Debraj ^{[1
]}

Bekki, Makoto ^{[2
]}

Nakamura, Masako ^{[2
]}

Sonezaki, Shuji ^{[2
]}

Ohji, Tatsuki ^{[1
]}

Kato, Kazumi ^{[1
]}

Kimura, Tatsuo ^{[1
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Adv Mfg Res Inst, Moriyama Ku, Nagoya, Aichi 4638560, Japan

[2] TOTO Ltd, Biotechnol Grp, Res Lab Nakashima, Kokurakita Ku, Kitakyushu, Fukuoka 8028601, Japan

来源：

JOURNAL OF MATERIALS CHEMISTRY | 2011年 / 21卷 / 15期

关键词：

TIO2; FILMS; MESOPOROUS SILICA; TITANIA FILMS; THIN-FILMS; FABRICATION; CRYSTAL; ARRAYS; PORES;

D O I：

10.1039/c0jm04347h

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Transparent semiconducting oxide (TiO2, SnO2 and ZnO) films having highly interconnected pores in the range of 30-200 nm were fabricated in one-pot using polystyrene-block-poly(ethylene oxide) (PS-b-PEO) diblock copolymer. Such porous structures were helpful for efficient transport of bulky guest species such as proteins. Biosensing protocols were constructed in the films by using bulky biomolecules, namely, adsorbed DNA, adsorbed antibody and adsorbed antigen-antibody (immunoassay). In the DNA-based system, the macroporous TiO2 film was the most useful among porous anatase films such as mesoporous and mesoporous/PS beads derived macroporous ones, leading to significant detection of 125 times higher photocurrent generated from a dye (Cy5) attached to DNA than those observed for the others. Macroporous SnO2 and ZnO films were also available for the construction of similar biomolecule immobilized electrodes. Extremely complicated immunoassay reactions involving bulky proteins (antigen, antibody, etc.) were also established using the macroporous TiO2 and SnO2 films. Especially photocurrent for electron transfer from Cy5 attached to the second antibody was successfully detected by using the SnO2 film for exceptionally selective sensing applications.

引用

页码：5738 / 5744

页数：7

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