Detailed organization of nitric oxide synthase, vasopressin and oxytocin immunoreactive cell bodies in the supraoptic nucleus of the female rat

被引:25
作者
Nylén, A
Skagerberg, G
Alm, P [1 ]
Larsson, B
Holmqvist, BI
Andersson, KE
机构
[1] Univ Lund Hosp, Dept Pathol, S-22185 Lund, Sweden
[2] Univ Lund Hosp, Dept Clin Pharmacol, S-22185 Lund, Sweden
[3] Univ Lund Hosp, Dept Neurosurg, S-22185 Lund, Sweden
来源
ANATOMY AND EMBRYOLOGY | 2001年 / 203卷 / 04期
关键词
neuroendocrine regulation; hypothalamo-neurohypophysial system; NADPH-diaphorase; immunocytochemistry; triple labelling;
D O I
10.1007/s004290100164
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
cell bodies containing neuronal nitric oxide synthase (nNOS), 8-arginine vasopressin (AVP) and oxytocin (OT) were examined throughout the supraoptic nucleus (SON) of the female rat by means of immunocytochemical and NADPH-diaphorase (NADPH-d) histochemical techniques using a triple labelling methodology. Seven chemically defined populations of neurons containing all combinations of either nNOS, AVP or OT were identified. nNOS-containing (NADPH-d positive) neurons, amounting to about 40% of all neurons counted, were most frequent in central and dorsal regions, and were evenly distributed along the rostro-caudal axis. Two small nNOS-positive neuronal populations were preferentially located dorso-centrally in the nucleus: nNOS-positive neurons containing both AVP- and OT-immunoreactivity, and neurons only containing nNOS. Slightly less than half of all nNOS-positive neurons contained AVP, and a similar share of nNOS-positive neurons contained OT. The occurrence of nNOS-positive/AVP-containing neurons was highest in the caudal half, whereas that of nNOS-positive/OT-neurons was highest in the rostral half of SON. The data demonstrate new findings concerning the anatomical organization and colocalization patterns of nNOS-, AVP- and OT-containing neuronal populations in SON. We conclude that the absolute and relative occurrence of the identified neuronal populations vary markedly in different parts of SON. This is important to take into consideration when performing, and evaluating experimental investigations concerned with neurochemical changes in SON.
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页码:309 / 321
页数:13
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