Capillary electrophoretic analysis of neutral carbohydrates using ionic liquids as background electrolytes

被引:39
作者
Vaher, Merike [1 ]
Koel, Mihkel [1 ]
Kazarjan, Jana [1 ]
Kaljurand, Mihkel [1 ]
机构
[1] Tallinn Univ Technol, Inst Chem, EE-12618 Tallinn, Estonia
关键词
Carbohydrates; CE; Imidazolium-based ionic liquids; Saccharides; Vegetable juices; CHROMATOGRAPHY; SEPARATION; DERIVATIZATION; ACETONITRILE; ETHANOL; SAMPLES; SUGARS; ACIDS;
D O I
10.1002/elps.201000575
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In this study, ionic liquids (ILs) as BGE additives were applied for the analysis of neutral carbohydrates in CE. The ILs served primarily as chromophores for indirect UV detection. The influence of imidazolium-based ionic liquids on the separation, detection limits and mobility of underivatized neutral carbohydrates was investigated. BGEs consisting of 10-50mM of ILs at pH 12.4 without other additives provided fast separation of neutral sugars. This method was used to determine sucrose, glucose and fructose in certain vegetable juices.
引用
收藏
页码:1068 / 1073
页数:6
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