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Comparison of culture and acid-fast bacilli stain to PCR for detection of Mycobacterium tuberculosis in clinical samples
被引:29
|作者:
Aslanzadeh, J
de la Viuda, M
Fille, M
Smith, WB
Namdari, H
机构:
[1] Univ Connecticut, Ctr Hlth, Dept Lab Med, Farmington, CT 06030 USA
[2] Inst Hyg, A-6020 Innsbruck, Austria
[3] Connecticut State Dept Publ Hlth, Hartford, CT USA
[4] Clin Labs Inc, Throop, PA USA
关键词:
D O I:
10.1006/mcpr.1998.0174
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
The major drawback in effective use of polymerase chain reaction (PCR) for detectinng Mycobacterium tuberculosis (MTB) in clinical samples is the presence of PCR inhibitors and unique cell components of the organism that complicate DNA extraction and subsequent PCR amplification. A PCR assay with a unique multistep DNA extraction method that minimizes these problems was compared in a prospective study to acid-fast bacilli stain (AFBS) and culture for detecting MTB in clinical samples. A total of 254 clinical specimens in two separate studies were processed for MTB by these techniques. While PCR and culture were 100% sensitive and specific, culture required up to 8 weeks of incubation and additional time to perform biochemical testing to identify the isolated micro-oganism. Acid-fast bacilli slain had a specificity of about 87% and did not differentiate among Mycobacterial species. In contrast, the results from PCR were available within 48 h and did not require additional testing to attain a final result. Polymerase chain reaction was highly reliable for detection and confirmation and interpretation of positive AFBS results. The assay was easy to perform with a turn around time of about 2 days. (C) 1998 Academic Press.
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页码:207 / 211
页数:5
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