Transgenic Expression of chit42 gene from Metarhiziumanisopliae in Trichoderma harzianum Enhances Antagonistic Activity against Botrytis cinerea

被引：5
作者：
Xia, H. [1 ,2 ]
Li, Y. Y. [1 ,2 ]
Liu, Z. C. [1 ,2 ]
Li, Y. Q. [1 ,2 ]
Chen, J. [1 ,2 ,3 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China
[2] Minist Agr, Key Lab Urban Agr South, Shanghai 200240, Peoples R China
[3] Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China
来源：
MOLECULAR BIOLOGY | 2018年 / 52卷 / 05期
基金：
中国国家自然科学基金;
关键词：
chit42; gene; Metarhizium anisopliae; Trichoderma harzianum; antagonistic activity; Botrytis cinerea; SECONDARY METABOLISM; CHITINASE CHIT42; ANTIFUNGAL; CLONING; MYCOPARASITISM; ANISOPLIAE; FUNGI;
D O I：
10.1134/S002689331805014X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Chitinases expressed by some beneficial fungi are crucial for the biocontrol of phytopathogens. The activity of chitinolytic strains of Trichoderma sp. may be enhanced by increasing the expression of chitinases. We describe the Trichoderma strain Mchit42 which expresses a transgenic chitinase chit42 from Metarhizium anisopliae. Inhibitory effects against plant pathogens were tested. Comparison of WT (T30) and OE (Mchit42) indicated that overexpression of M. anisopliaechit42 did not alter Trichoderma growth, while enhancing the expression of endogenous chitinase, -1,3-glucanases, and polygalacturonase and increasing the antagonistic activity of Trichoderma against Botrytis cinerea. This work confirmed that the expression of the entomopathogenic fungi-sourced chit42 genes in Trichodermaharzianum enhances the efficiency of Trichoderma biocontrol against targeted pathogens.
引用
收藏
页码：668 / 675
页数：8
相关论文
共 31 条
  • [1] Microsatellite loci from Russula brevipes, a common ectomycorrhizal associate of several tree species in North America
    Bergemann, SE
    Miller, SL
    Garbelotto, M
    [J]. MOLECULAR ECOLOGY NOTES, 2005, 5 (03): : 472 - 474
  • [2] Synergistic activity of endochitinase and exochitinase from Trichoderma atroviride (T-harzianum) against the pathogenic fungus (Venturia inaequalis) in transgenic apple plants
    Bolar, JP
    Norelli, JL
    Harman, GE
    Brown, SK
    Aldwinckle, HS
    [J]. TRANSGENIC RESEARCH, 2001, 10 (06) : 533 - 543
  • [3] Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method
    Bonev, Boyan
    Hooper, James
    Parisot, Judicael
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2008, 61 (06) : 1295 - 1301
  • [4] Characterization of the procera Tomato Mutant Shows Novel Functions of the SlDELLA Protein in the Control of Flower Morphology, Cell Division and Expansion, and the Auxin-Signaling Pathway during Fruit-Set and Development
    Carrera, Esther
    Ruiz-Rivero, Omar
    Pereira Peres, Lazaro Eustaquio
    Atares, Alejandro
    Luis Garcia-Martinez, Jose
    [J]. PLANT PHYSIOLOGY, 2012, 160 (03) : 1581 - 1596
  • [5] Isolation of a new fungi and wound-induced chitinase class in corms of Crocus sativus
    Castillo Lopez, Raquel
    Gomez-Gomez, Lourdes
    [J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2009, 47 (05) : 426 - 434
  • [6] Chet I., 1997, MYCOTA
  • [7] PLANT CHITINASES
    COLLINGE, DB
    KRAGH, KM
    MIKKELSEN, JD
    NIELSEN, KK
    RASMUSSEN, U
    VAD, K
    [J]. PLANT JOURNAL, 1993, 3 (01) : 31 - 40
  • [8] Cumagun CJR, 2012, J AGR SCI TECH-IRAN, V14, P699
  • [9] Cuticle-induced endo/exoacting chitinase CHIT30 from Metarhizium anisopliae is encoded by an ortholog of the chi3 gene
    da Silva, MV
    Santi, L
    Staats, CC
    da Costa, AM
    Colodel, EM
    Driemeier, D
    Vainstein, MH
    Schrank, A
    [J]. RESEARCH IN MICROBIOLOGY, 2005, 156 (03) : 382 - 392
  • [10] Faheem Amin Faheem Amin, 2010, Journal of Phytology, V2, P38
1234