Comparative pharmacokinetics of acteoside from total glycoside extracted from leaves of Rehmannia and Dihuangye total glycoside capsule in normal and diabetic nephropathy rats

被引：16

作者：

Dai, Xin-Xin ^{[1
,2
]}

Su, Shu-Lan ^{[1
,2
]}

Cai, Hong-Die ^{[1
,2
]}

Wei, Dan-Dan ^{[1
,2
]}

Zheng, Tian-Yao ^{[1
,2
,3
]}

Zhu, Zhen-Hua ^{[1
,2
]}

Yan, Hui ^{[1
,2
]}

Shang, Er-Xin ^{[1
,2
]}

Guo, Sheng ^{[1
,2
]}

Qian, Da-Wei ^{[1
,2
]}

Duan, Jin-Ao ^{[1
,2
,4
]}

机构：

[1] Nanjing Univ Chinese Med, Jiangsu Collaborat Innovat Ctr Chinese Med Resour, Natl & Local Collaborat Engn Ctr, Chinese Med Resources Ind & Formulae Innovat Med, Nanjing, Jiangsu, Peoples R China

[2] Nanjing Univ Chinese Med, Jiangsu Key Lab High Technol Res TCM Formulae, Nanjing, Jiangsu, Peoples R China

[3] Jiaugsu Univ, Sch Pharm, Zhenjiang, Peoples R China

[4] Nanjing Univ Chinese Med, State Key Lab Cultivat Base TCM Qual & Efficacy, Nanjing, Jiangsu, Peoples R China

来源：

BIOMEDICAL CHROMATOGRAPHY | 2017年 / 31卷 / 12期

基金：

中国国家自然科学基金;

关键词：

acteoside; diabetic nephropathy; pharmacokinetics; Rehmannia glutinosa; GINSENOSIDE RB1; GLUTINOSA; METABOLISM; OLIGOSACCHARIDES; MICROFLORA; MICROBIOTA; MECHANISM; MODEL;

D O I：

10.1002/bmc.4013

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Rehmannia glutinosa Libosch (RG), is officially listed in the Chinese Pharmacopoeia and is widely used in China. In this paper, a sensitive and rapid ultra-performance liquid chromatography-mass spectrometry method including multiple-reaction monitoring mode was developed and applied to study the pharmacokinetic effect of acteoside from total glycoside extracted from the leaves of Rehmannia (TLR) and Dihuangye total glycoside capsule (DTG) in normal and diabetic nephropathy rats. The diabetic nephropathy rat model was induced by intraperitoneal injection of a small dose of streptozotocin and high-fat diet and plus 5% glucose drinking water. Samples of plasma of rats were obtained at different times after rats were administered TLR (7.2g/kg) and DTG (360mg/kg). After deproteinization by acetonitrile, the concentrations of acteoside in rats at different time points were detected by UPLC-TQ-MS method and pharmacokinetics parameters were calculated using DAS 3.2.8 software. A good linearity of acteoside was shown in the range of 8.51-3404.8ng/m L (r(2)=0.9987). The mean extraction recovery of analyte was in the range of 63.55-79.49%, and the intra- and inter-day RSD values were <8.8%. Compared with the normal group, the maximum plasma concentration, AUC(0-t), AUC(0-) and apparent plasma clearance corresponding dose in model group rats decreased significantly. After rats were administered TLR and DTG, the acteoside reached the maximum plasma concentration at about 15min. The method proved to be simple, rapid and specific, and to be suitable for the determination of acteoside in plasma of diabetic nephropathy rats and pharmacokinetic study.

引用

页数：7

共 35 条

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