The diagnostic performance and accuracy of 3 molecular assays for the detection of Clostridium difficile in stool samples, compared with the Xpert® C. difficile assay

被引：3

作者：

Azrad, Maya ^{[1
]}

Tkhawkho, Linda ^{[1
]}

Hamo, Zohar ^{[1
]}

Peretz, Avi ^{[1
,2
]}

机构：

[1] Baruch Padeh Med Ctr, Clin Microbiol Lab, Poriya, Tiberias, Israel

[2] Bar Ilan Univ, Azrieli Fac Med, Safed, Israel

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2020年 / 168卷

关键词：

Clostridium difficile; Performance; Diagnostic accuracy; Molecular assays; BD MAX CDIFF; UNIVERSAL DIRECT; SIMPLEXA; TESTS;

D O I：

10.1016/j.mimet.2019.105784

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This research compares the performance and diagnostic accuracy of three molecular tests for the detection of Clostridium difficile in stool samples, with the Xpert (R) C. difficile assay. Fifty-nine positive and twenty-five stool negative samples were analyzed by the BDmax (TM) Cdiff, the Simplexa C. difficile Direct, and the GenomEra (TM) C. difficile, and compared with the Xpert (R) C. difficile routinely used in our laboratory. The highest sensitivity was 94.9% for the BDmax (TM) Cdiff, followed by GenomEre (TM) C. difficile with 93.2%, and Simplexa (TM) C. difficile Direct with 89.8%. The specificities of all assays were 100%. GenomEra (TM) C. difficile had the highest retesting rate (12%). Simplexa (TM) C. difficile Direct benefits from both short hands-on time and total-turnaround time and 0% retesting. The differences in performance and accuracy between these three molecular assays are insignificant and all may be used as part of the routine algorithm for detection of C. difficile.

引用

页数：4

共 20 条

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