A study of HAp/PLLA composite as a substitute for bone powder, using FT-IR spectroscopy

被引:115
作者
Ignjatovic, N
Savic, V
Najman, S
Plavsic, M
Uskokovic, D
机构
[1] Serbian Acad Arts & Sci, Inst Tech Sci, YU-11000 Belgrade, Yugoslavia
[2] Univ Nis, Fac Med, YU-18000 Nis, Yugoslavia
[3] Fac Technol & Met Belgrade, YU-11000 Belgrade, Yugoslavia
关键词
hydroxyapatite/poly-L-lactide composite biomaterial; in vivo; FT-IR spectroscopy; absorption band; collagen; autologous bone;
D O I
10.1016/S0142-9612(00)00215-5
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Chemically synthesized hydroxyapatite/poly-L-lactide (HAp/PLLA) composite biomaterial was studied in vivo. The biocomposite was implanted into Balb/c Singen mice and after 1 and 3 weeks removed from their organisms and analyzed by the FT-IR spectroscopy. After 1 week of testing in vivo the implanted sample gave a spectrum in which absorption bands arising from newly formed functional groups of amine and peptide can be seen. After 3 weeks, a spectrum with pronounced absorption bands at 3420 and 1650 cm(-1) assigned to newly generated collagen, a component of the extracellular connective-tissue matrix, was registered. Also, decrease of the intensity absorption band at 1760 cm(-1) originating from the C=O group of PLLA indicates bioresorption of the PLLA used. Analysis of the microstructure of the sample surface by scanning electron microscopy before and after implantation revealed bioresorption of the PLLA polymer phase and generation of collagen fibers at the sites of implanted bioresorptive PLLA. A mixture of autologous bone powder and HAp/PLLA biocomposite was also examined. After implantation, the same final products as in the case of HAp/PLLA composite biomaterial used alone were found. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:571 / 575
页数:5
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