Complex target SELEX-based identification of DNA aptamers against Bungarus caeruleus venom for the detection of envenomation using a paper-based device

被引：18

作者：

Anand, Anjali ^{[1
]}

Chatterjee, Bandhan ^{[1
]}

Dhiman, Abhijeet ^{[1
]}

Goel, Renu ^{[2
]}

Khan, Eshan ^{[3
]}

Malhotra, Anita ^{[4
]}

Santra, Vishal ^{[5
,6
]}

Salvi, Nitin ^{[7
]}

Khadilkar, M., V ^{[7
]}

Bhatnagar, Ira ^{[8
]}

Kumar, Amit ^{[3
]}

Asthana, Amit ^{[8
,9
]}

Sharma, Tarun Kumar ^{[1
]}

机构：

[1] Translat Hlth Sci & Technol Inst THSTI, Aptamer Technol & Diagnost Lab, Multidisciplinary Clin & Translat Res Grp, Faridabad 121001, Haryana, India

[2] Translat Hlth Sci & Technol Inst THSTI, Faridabad 121001, Haryana, India

[3] Indian Inst Technol Indore, Discipline Biosci & Biomed Engn, Indore 453552, India

[4] Bangor Univ, Coll Environm Sci & Engn, Sch Nat Sci, Bangor LL57 2UW, Gwynedd, Wales

[5] Soc Nat Conservat Res & Community Engagement, Hooghly 712407, W Bengal, India

[6] Capt & Field Herpetol Ltd, 13 Hirfron, Llaingoch LL65 1YU, Anglesey, Wales

[7] Premium Serums & Vaccines Pvt Ltd, Junnar 410504, Maharashtra, India

[8] CSIR Ctr Cellular & Mol Biol, CCMB Annexe 2,Med Biotechnol Complex,Uppal Rd, Hyderabad 500039, Telangana, India

[9] Natl Inst Pharmaceut Educ & Res NIPER, Dept Pharmaceut Anal, Hyderabad 500037, Telangana, India

来源：

BIOSENSORS & BIOELECTRONICS | 2021年 / 193卷

关键词：

Aptamers; SELEX; Krait; Snake venom; Bungarotoxin; Mass-spectrometry; Paper device; SNAKE-VENOM; BIOMARKER DISCOVERY; SRI-LANKA; ANTIBODIES; DIAGNOSIS; PROFILES; DISEASE; INDIA;

D O I：

10.1016/j.bios.2021.113523

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Complex target SELEX always have been an intriguing approach to the scientific community, as it offers the potential discovery of novel biomarkers. We herein successfully performed SELEX on Bungarus caeruleus venom to develop a panel of highly affine aptamers that specifically recognizes the B. caeruleus (common krait) venom and was able to discriminate the B. caeruleus venom from Cobra, Russell's, and Saw-scaled viper's venom. The aptamers generated against the crude venom also lead to the identification of the specific component of the venom, which is beta-Bungarotoxin, a toxin uniquely present in the B. caeruleus venom. The best performing aptamer candidates were used as a molecular recognition element in a paper-based device and were able to detect as low as 2 ng krait venom in human serum background. The developed aptamer-based paper device can be used for potential point-of-care venom detection applications due to its simplicity and affordability.

引用

页数：12

共 74 条

[1]

A.A, 2013, J. Chem. Inf. Model, V53, P1689, DOI [DOI 10.1017/CBO9781107415324.004, 10.1017/CBO9781107415324.004]

[2]

[Anonymous], 1999, Southeast Asian J Trop Med Public Health, V30 Suppl 1, P1

[3]

[Anonymous], 2012, J CLIN INVEST, V122, P1734

[4] Syndromic Approach to Treatment of Snake Bite in Sri Lanka Based on Results of a Prospective National Hospital-Based Survey of Patients Envenomed by Identified Snakes [J].

Ariaratnam, Christeine A. ;

Sheriff, Mohamed H. Rezvi ;

Arambepola, Carukshi ;

Theakston, R. David G. ;

Warrell, David A. .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2009, 81 (04) :725-731

[5] Aptamer-facilitated biomarker discovery (AptaBiD) [J].

Berezovski, Maxim V. ;

Lechmann, Matthias ;

Musheev, Michael U. ;

Mak, Tak W. ;

Krylov, Sergey N. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (28) :9137-9143

[6]

Bonin-Debs AL, 2004, EXPERT OPIN BIOL TH, V4, P551, DOI 10.1517/eobt.4.4.551.29462

[7]

Brunda G, 2006, J AOAC INT, V89, P1360

[8] Causes and Consequences of Snake Venom Variation [J].

Casewell, Nicholas R. ;

Jackson, Timothy N. W. ;

Laustsen, Andreas H. ;

Sunagar, Kartik .

TRENDS IN PHARMACOLOGICAL SCIENCES, 2020, 41 (08) :570-581

[9]

Chadwick S.J., 2008, Managing the Allergic Patient, P19, DOI [10.1016/B978-1-4160-3677-7.X5001-8, DOI 10.1016/B978-1-4160-3677-7.X5001-8]

[10]

Chatterjee B., 2020, Materials Science for Energy Technologies, V3, P127, DOI [10.1016/j.mset.2019.08.007, DOI 10.1016/J.MSET.2019.08.007]

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