Apoptosis as a Possible Candidate Mechanism for Removal of Tamoxifen-related Endometrial Cells with KRAS Mutations

被引:0
作者
Tsujioka, Hiroshi [1 ]
Hachisuga, Toru [2 ]
Hikita, Shoko
Ueda, Taeko
Yotsumoto, Fusanori [3 ]
Shirota, Kyoko
Yoshizato, Toshiyuki
Kawarabayashi, Tatsuhiko
Kuroki, Masahide [3 ]
Miyamoto, Shingo
机构
[1] Fukuoka Univ, Fac Med, Dept Obstet & Gynecol, Jonan Ku, Fukuoka 8140180, Japan
[2] Univ Occupat & Environm Hlth, Dept Obstet & Gynecol, Kitakyushu, Fukuoka 807, Japan
[3] Fukuoka Univ, Fac Med, Dept Biochem, Fukuoka 8140180, Japan
关键词
Tamoxifen; endometrium; KRAS; apoptosis; K-RAS MUTATIONS; BREAST-CANCER PATIENTS; DNA-ADDUCTS; MICROSATELLITE INSTABILITY; ADJUVANT TAMOXIFEN; CARCINOGENESIS; HYPERPLASIA; CARCINOMAS; TOREMIFENE; ACTIVATION;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: Endometrial cell KRAS mutations are frequent in tamoxifen (TAM)-treated breast cancer patients. We previously demonstrated that most KRAS mutations disappeared after TAM cessation, suggesting the existence of a removal mechanism for endometrial cells with KRAS mutation. Here, the role of apoptosis in this mechanism was investigated. Patients and Methods: DNA was extracted from frozen endometrial polyps of 31 TAM-treated breast cancer patients. Codon 12 mutations in KRAS were detected by enriched polymerase chain reaction enzyme-linked minisequence assay. Apoptosis was detected by the TdT-mediated dUTP-biotin nick end-labeling (TUNEL) method and Ki-67 expression by immunohistochemistry. Relationships between KRAS mutations, the apoptosis index, and the Ki-67 index were determined. Results: KRAS mutations were observed in 9 of these patients. There was no significant relationship between the Ki-67 index and KRAS mutation. However, the apoptosis index was significantly higher in polyps with KRAS mutation (p=0.002). Conclusion: Apoptosis may play an important role in removing TAM treatment-related endometrial cells with KRAS mutations.
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收藏
页码:3119 / 3123
页数:5
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