Using intrinsic fluorescence to investigate proteins entrapped in sol-gel derived materials

被引：68
作者：
Brennan, JD [1 ]
机构：
[1] McMaster Univ, Dept Chem, Hamilton, ON L8S 4M1, Canada
来源：
APPLIED SPECTROSCOPY | 1999年 / 53卷 / 03期
关键词：
D O I：
10.1366/0003702991946514
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
An account is given of the recent efforts involving the use of fluorescence spectroscopy to elucidate subtle features of entrapped proteins, including structure, conformational flexibility, accessibility to analyte, stability, and function. The particular areas where spectroscopic methods benefit the study of entrapped proteins are highlighted, and the overall utility of fluorescence spectroscopy for studies of entrapped proteins is discussed. The use of fluorescence to examine factors important to sensor development is also detailed.
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页码：106A / 121A
页数：16
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