NADPH oxidase involvement in cellular integrity

被引：31

作者：

Macpherson, Neil ^{[1
]}

Takeda, Seiji ^{[2
]}

Shang, Zhonglin ^{[1
]}

Dark, Adeeba ^{[1
]}

Mortimer, Jennifer C. ^{[1
]}

Brownlee, Colin ^{[3
]}

Dolan, Liam ^{[2
]}

Davies, Julia M. ^{[1
]}

机构：

[1] Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England

[2] John Innes Inst, Dept Cell & Dev Biol, Norwich NR4 7UH, Norfolk, England

[3] Marine Biol Assoc UK, Plymouth PL2 3PB, Devon, England

来源：

PLANTA | 2008年 / 227卷 / 06期

基金：

英国自然环境研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

cell wall; NADPH oxidase; osmoregulation; reactive oxygen species; rhd2; root hair;

D O I：

10.1007/s00425-008-0716-2

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

NADPH oxidase activity is involved in plant adaptation and development. The reactive oxygen species sourced by NADPH oxidase activity may contribute to wall strength and protoplast volume adjustment. Root hair bulge apices of the NADPH oxidase mutant rhd2/Atrbohc were more robust than the kjk cellulose synthase mutant, but burst more readily than the wild type (WT). Root epidermal wall appeared impaired in rhd2/Atrbohc, as revealed by the number of protoplasts released by wall-degrading enzymes. Root hair bulges of rhd2/Atrbohc burst more than the WT when challenged in situ with hypo-osmotic low ionic strength medium. Inhibition of NADPH oxidase activity with diphenylene iodonium caused WT to phenocopy the rhd2/Atrbohc bursting in response to hypo-osmotic shock. This implicates RHD2/AtRBOHC in softening the cell wall to permit protoplast expansion. Overall, the results point to a role for RHD2/AtRBOHC in contributing to wall strength.

引用

页码：1415 / 1418

页数：4

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