Rapid enhanced tissue culture immunofluorescence: a new, rapid method for detection of viruses

被引：1

作者：

Alexander, R ^{[1
]}

Lamb, D ^{[1
]}

机构：

[1] Royal Childrens Hosp, Dept Microbiol & Infect Dis, Womens & Childrens Healthcare Network, Melbourne, Vic 3052, Australia

来源：

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS | 2003年 / 3卷 / 02期

关键词：

rapid enhanced tissue culture immunofluorescence; rapid virus detection;

D O I：

10.1586/14737159.3.2.249

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

The detection of a large variety of viruses requires a number of different susceptible cell lines in a shell vial method - currently considered to be the most enhanced method of viral detection. However, this method is unsuitable in its standard form for handling a large volume of specimens. We have developed a time and cost saving now method, named rapid enhanced tissue culture immunofluorescence (RETCIF) and incorporates the attributes of the current methods. It is specific, uses the most sensitive cell lines and monoclonal antibodies and does not require the use of cover slips. The RETCIF method, in our hands, is a time saving procedure, with higher isolation ratio than the shell vial method. We recommend the RETCIF method for busy diagnostic virology laboratories.

引用

页码：249 / 263

页数：15

共 33 条

[1] 'RETCIF': a rapid, sensitive method for detection of viruses, applicable for large numbers of clinical samples [J].