Glycomyces halotolerans sp nov., a novel actinomycete isolated from a hypersaline habitat in Xinjiang, China

A novel actinomycete strain, designated TRM 40137(T), was isolated from a hypersaline habitat in Xinjiang Province, north-west China, and subjected to a polyphasic taxonomic study. The strain was aerobic, Gram-positive and the optimum NaCl concentration for growth was 4-5% (w/v). Phylogenetic analysis showed that strain TRM 40137(T) has a 16S rRNA gene sequence similarity of 95.02% with the described species Glycomyces sambucus E71(T) and can be distinguished from all previously described representatives of the genus Glycomyces. The whole-cell sugar pattern consisted of xylose and galactose. The predominant menaquinone was MK-10(H-2) and the major fatty acids were anteiso-C15:0 and anteiso-C17:0. The phospholipid pattern consists of phosphatidylglycerol, diphosphatidylglycerol, three unknown aminophospholipids and two unknown phospholipids. The G+C content of the genomic DNA was 68.8 mol%. A novel species Glycomyces halotolerans sp. nov. is proposed, with strain TRM 40137(T) (=CCTCC AA 2010013(T) = KCTC 19988(T)) as the type strain of G. halotolerans.