Sdmg1 is a conserved transmembrane protein associated with germ cell sex determination and germline-soma interactions in mice

被引:63
作者
Best, Diana [1 ]
Sahlender, Daniela A. [2 ]
Walther, Norbert [3 ]
Peden, Andrew A. [2 ]
Adams, Ian R. [1 ,4 ,5 ]
机构
[1] Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland
[2] Univ Cambridge, Inst Med Res, Cambridge CB2 2XY, England
[3] Univ Hamburg, Sch Life Sci, D-22527 Hamburg, Germany
[4] Univ Edinburgh, Sch Mol & Clin Med, Canc Res Ctr, Western Gen Hosp, Edinburgh EH4 2XU, Midlothian, Scotland
[5] Univ Cambridge, Gurdon Inst, Cambridge CB2 1QN, England
来源
DEVELOPMENT | 2008年 / 135卷 / 08期
基金
英国惠康基金; 英国医学研究理事会;
关键词
mouse; primordial germ cells; sertoli cell; membrane trafficking; sdmg1 (Tmem184a);
D O I
10.1242/dev.019497
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In mammals, the supporting cell lineage in an embryonic gonad communicates the sex-determining decision to various sexually dimorphic cell types in the developing embryo, including the germ cells. However, the molecular nature of the sex-determining signals that pass from the supporting cells to the germ cells is not well understood. We have identified a conserved transmembrane protein, Sdmg1, owing to its male-specific expression in mouse embryonic gonads. Sdmg1 is expressed in the Sertoli cells of embryonic testes from 12.5 dpc, and in granulosa cells of growing follicles in adult ovaries. In Sertoli cells, Sdmg1 is localised to endosomes, and knock-down of Sdmg1 in Sertoli cell lines causes mis-localisation of the secretory SNARE Stx2 and defects in membrane trafficking. Upregulation of Sdmg1 appears to be part of a larger programme of changes to membrane trafficking pathways in embryonic Sertoli cells, and perturbing secretion in male embryonic gonads in organ culture causes male-to-female germ cell sex reversal. These data suggest that changes that occur in the cell biology of embryonic Sertoli cells may facilitate the communication of male sex-determining decisions to the germ cells during embryonic development.
引用
收藏
页码:1415 / 1425
页数:11
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